Anti-NF-kB p65/RelA Rabbit Polyclonal Antibody for WB - Q04206

Core Product Specifications and Parameters

Biological Background: NF-kB p65/RelA Function and Localization

• NF-kB p65/RelA, encoded by the RELA gene, is a subunit of the NF-kB transcription factor complex that exists as homo- or heterodimers; the heterodimeric p65-NFKB1 complex is the most abundant form.
• It plays a central role in inflammation, immunity, cell growth, differentiation, and apoptosis by binding to kappa-B sites in target gene promoters, acting primarily as a transcriptional activator.
• Among NF-kB family members (RELA, RELB, REL, NFKB1, NFKB2), p65/RelA is essential for cytokine gene expression in T-cells (PubMed:15790681). Related references: PMID:15790681
• The subcellular localization of p65 is tightly regulated; in resting cells, it is sequestered in the cytoplasm by IκB inhibitors, and upon activation, it translocates to the nucleus.
• p65/RelA is subject to multiple post-translational modifications including phosphorylation, acetylation, methylation, and ubiquitination, which modulate its transcriptional activity and stability.
• It is also involved in host-virus interactions, acting as a key transcription factor regulating the interferon response during SARS-CoV-2 infection (PubMed:33440148). Related references: PMID:33440148
• The protein has a predicted molecular weight of approximately 60 kDa (UniProt: 60219 Da) and is expressed in nearly all cell types.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal transactivation domain (aa 450-551) of human p65/RelA; antibody reactivity may be influenced by post-translational modifications in this region.
• For Western blotting, a band around 60 kDa is expected; consider including appropriate positive and negative controls (e.g., stimulated vs. unstimulated cells) to confirm specificity.
• In immunoprecipitation, the polyclonal nature may provide robust capture; validate in the relevant sample system to confirm efficient pulldown of the p65 complex.
• For immunofluorescence/ICC, anticipate nuclear translocation upon NF-κB pathway activation; use fixation and permeabilization conditions suitable for nuclear antigens.
• This antibody is recommended for ELISA; verify optimal coating and detection conditions with recombinant p65 or cell lysates.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and laboratories with integrated access to high-quality IVD raw materials, including validated antibody pairs and bulk ancillary reagents. For NF-κB signaling and inflammation research, CamelBio offers customized sourcing solutions for polyclonal antibodies like this anti-p65/RelA, along with technical consulting from concept to clinic. Explore our one-stop platform to streamline your raw material procurement.