Anti-NF-kB p65/RelA Mouse Monoclonal Antibody for WB, IF/ICC, ELISA - Q04206

Core Product Specifications and Parameters

Biological Background: NF-kB p65/RelA Function and Localization

• Transcription factor p65, also known as RELA, nuclear factor NF-kappa-B p65 subunit, encoded by the RELA gene (NFKB3).
• NF-kappa-B is a pleiotropic transcription factor present in almost all cell types, involved in inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis.
• Forms homo- or heterodimers with Rel domain proteins; the heterodimer with NFKB1/p50 is the most abundant, acting as a transcriptional activator.
• Subcellular location is both nucleus and cytoplasm; inactive complexes are retained in the cytoplasm via interaction with I-kappa-B inhibitors, and upon activation, translocate to the nucleus.
• Subject to multiple post-translational modifications including acetylation, phosphorylation, methylation, and ubiquitination, which regulate its activity.
• Essential for cytokine gene expression in T-cells (PubMed:15790681). Related references: PMID:15790681
• Key transcription factor regulating the IFN response during SARS-CoV-2 infection (PubMed:33440148). Related references: PMID:33440148

Experimental Guidance and Technical Tips

• Consider optimizing antibody dilution for each application and sample type. For Western blot, a band at approximately 60 kDa is expected.
• Validate specificity using knockdown or knockout models if possible.
• In immunocytochemistry, fixation and permeabilization conditions may influence staining; inclusion of appropriate controls is recommended.
• The antibody may be suitable for ELISA, but pairing with a detection antibody should be empirically determined.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop partner for IVD raw material procurement, offering validated monoclonal and polyclonal antibodies, optimized antibody pairs, and bulk ancillary reagents to support your assay development. Our expertise in NF-kB signaling and inflammation targets ensures you can source high-quality reagents for reliable diagnostic and research applications. Let us streamline your supply chain from concept to clinic.