Anti-NF-kB p65/RelA Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA, ChIP - Q04206

Core Product Specifications and Parameters

Biological Background: NF-kB p65/RelA Function and Localization

• The NF-kB p65 subunit (also known as RELA, NFKB3) is a core component of the NF-kappa-B transcription factor complex, a pleiotropic regulator present in almost all cell types.
• NF-kappa-B complexes are homo- or heterodimers formed by REL-like domain-containing proteins; the heterodimer of RELA and NFKB1 (p50) is the most abundant form and acts as a transcriptional activator.
• RELA/p65 plays a central role in inflammatory and immune responses, as well as in cell differentiation, proliferation, apoptosis, and tumorigenesis, by binding to kappa-B sites in target gene promoters.
• In unstimulated cells, NF-kappa-B dimers are retained in the cytoplasm by inhibitors (I-kappa-B); upon activation, I-kappa-B is phosphorylated and degraded, allowing RELA to translocate to the nucleus to regulate gene expression.
• RELA is subject to extensive post-translational modifications, including acetylation, phosphorylation, methylation, S-nitrosylation, and ubiquitination, which fine-tune its activity, stability, and interactions.
• RELA is essential for cytokine gene expression in T-cells, as demonstrated by functional studies. Related references: PMID:15790681
• RELA has been identified as a key transcription factor regulating the interferon response during SARS-CoV-2 infection. Related references: PMID:33440148
• The protein is primarily localized to the nucleus and cytoplasm, with dynamic shuttling between these compartments depending on activation status.

Experimental Guidance and Technical Tips

• For Western blot (WB), a specific band around 60 kDa is expected, consistent with the calculated molecular weight of NF-kB p65.
• For IHC-P and IF/ICC, validation in the relevant tissue or cell system is recommended; antigen retrieval may be required for paraffin-embedded sections.
• The KO validation data indicate specificity for endogenous RELA; users are advised to include appropriate controls (e.g., knockout cell lysates) in their experiments.
• In ChIP assays, the antibody recognizes native NF-kB p65 bound to chromatin; optimal fixation and shearing conditions should be determined empirically.
• Although the antibody is listed for multiple applications, individual assay conditions (e.g., dilution, incubation time) should be optimized in the end-user's laboratory.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents to diagnostic manufacturers and research institutions. Our supply capabilities cover critical targets like NF-kB p65 to support inflammation, oncology, and infectious disease assay development. From concept to clinic, CamelBio provides one-stop access to rare target raw-material sourcing backed by technical expertise.