Anti-NEXN Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q0ZGT2

Core Product Specifications and Parameters

Biological Background: NEXN Function and Localization

• Nexilin (NEXN), also known as Nelin or CMH20, is an F-actin-binding protein that plays a critical role in the maintenance of Z-line and sarcomere integrity in striated muscle.
• It regulates cell migration by directly associating with the actin cytoskeleton, influencing cytoskeletal dynamics and adhesion.
• The protein contains immunoglobulin-like domains and undergoes alternative splicing, producing multiple isoforms with tissue-specific distributions.
• Subcellularly, NEXN localizes to the cytoplasm, cytoskeleton, cell–cell adherens junctions, and the sarcomeric Z-line of myofibrils.
• Post-translational modifications include phosphorylation (phosphoprotein), which may modulate its actin-binding activity or localization.
• Tissue expression is highest in heart and skeletal muscle, with lower levels detected in placenta, lung, liver, pancreas, and certain cancer cell lines (HeLaS3, MOLT-4).
• Genetic variants in NEXN are associated with dilated cardiomyopathy and other cardiac diseases, highlighting its essential role in cardiac muscle function.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 516–675), which is conserved across human, mouse, and rat NEXN; consider this when designing species-specific detection panels.
• For Western blot, a band near 81 kDa is expected in heart and skeletal muscle lysates; validate the antibody in these tissues along with a negative control (e.g., NEXN knockout or siRNA-treated cells).
• In immunofluorescence/ICC, fixation and permeabilization conditions should be optimized to preserve the delicate sarcomeric Z-line and junctional structures. Co-staining with actin or Z-line markers may confirm localization.
• ELISA applications may require purified recombinant NEXN or peptide coating; consider titration and direct binding assays to establish working concentrations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, labs, and research institutes with integrated access to high-quality IVD raw materials. For NEXN-related projects, we offer validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. Our platform streamlines procurement for cardiac and skeletal muscle research, including rare-target raw-material sourcing, enabling you to move from concept to clinic efficiently.