Anti-Neutrophil Elastase (ELANE) Rabbit Polyclonal Antibody for WB, IF, IHC, ELISA - P08246

Core Product Specifications and Parameters

Biological Background: Neutrophil Elastase (ELANE) Function and Localization

• ELANE is primarily expressed in bone marrow cells and neutrophils (PubMed:10947984).
  Related references: PMID:10947984
• It modifies the functions of natural killer cells, monocytes, and granulocytes; inhibits C5a-dependent neutrophil enzyme release and chemotaxis (PubMed:15140022).
  Related references: PMID:15140022
• Exhibits microbicidal activity by mediating the cleavage and degradation of bacterial proteins; kills E. coli but not S. aureus in vitro; digests outer membrane protein A (OmpA) in E. coli and K. pneumoniae (PubMed:10947984, PubMed:25161283).
  Related references: PMID:10947984, PMID:25161283
• Critical for the formation of neutrophil extracellular traps (NETs): translocates to the nucleus downstream of MPO and degrades histone H4, promoting chromatin decondensation (PubMed:20974816, PubMed:25066128, PubMed:28574339).
  Related references: PMID:20974816, PMID:25066128, PMID:28574339
• Promotes blood coagulation and platelet aggregation by inactivating tissue factor pathway inhibitor (TFPI) and activating the fibrinogen receptor integrin alpha-IIb/beta-3 (PubMed:20676107, PubMed:25211214, PubMed:9111081).
  Related references: PMID:20676107, PMID:25211214, PMID:9111081
• Inhibits pyroptosis by promoting the cleavage of GSDMB (PubMed:36899106).
  Related references: PMID:36899106
• Subcellular localization includes cytoplasmic vesicles, phagosomes, lysosomes, and cytolytic granules; actively translocates to the nucleus during NETosis.
• Synthesized as a glycosylated preproenzyme; activated by proteolytic removal of the propeptide; stabilized by disulfide bonds.

Experimental Guidance and Technical Tips

• For Western blotting, the mature enzyme has a predicted molecular weight of ~29 kDa; however, the proform and glycosylation may lead to higher bands. Validate the observed band with appropriate controls.
• For immunohistochemistry (IHC) and immunofluorescence (IF), neutrophil-rich tissues such as bone marrow or inflamed tissue sections are recommended as positive controls. Note that ELANE can be secreted and may show diffuse staining.
• When using for ELISA, consider this polyclonal antibody as a capture or detection reagent. Pairing with a monoclonal antibody against a non-overlapping epitope may improve assay specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research laboratories by providing integrated access to IVD raw materials, technical services, and consulting support. For assays targeting neutrophil elastase and related inflammatory or NETosis pathways, we offer validated antibody pairs, optimized polyclonal antibodies, bulk ancillary reagents, and customized sourcing for rare targets. From concept to clinic, CamelBio is your reliable partner in accelerating assay development.