Anti-Neuropilin-2/NRP2 Rabbit Monoclonal Antibody for WB, FC, ELISA - O60462

Core Product Specifications and Parameters

Biological Background: Neuropilin-2 Function and Localization

• Neuropilin-2 (NRP2) is a membrane-bound co-receptor classically involved in semaphorin and vascular endothelial growth factor (VEGF) signaling.
• It functions as a high-affinity receptor for semaphorins 3C and 3F, the VEGF-165 and VEGF-145 isoforms, and the PLGF-2 isoform of PGF, mediating axon guidance, angiogenesis, and lymphangiogenesis.
• NRP2 acts as a receptor for human cytomegalovirus (HCMV) pentamer-dependent entry into epithelial and endothelial cells, facilitating viral infection.
• The protein is encoded by the NRP2 gene and exists in alternatively spliced isoforms, contributing to diverse biological functions.
• Subcellularly, NRP2 is localized to the plasma membrane and is also found as a secreted form, reflecting its dual membrane-bound and soluble signaling roles.
• Post-translational modifications include N-linked glycosylation, disulfide bond formation, and calcium/heparin binding, which are critical for ligand interaction and structural stability.
• The protein contains characteristic MAM, coagulation factor 5/8 type C, and complement binding domains, underscoring its classification within the neuropilin family.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full extracellular domain (aa 1-855), so the antibody likely recognizes all glycosylated and cleaved forms of NRP2; validate in the relevant sample system.
• For Western blot (WB), expect a band around 105 kDa (theoretical molecular weight), but note that glycosylation may result in a higher apparent molecular weight (~130-140 kDa).
• In flow cytometry (FC), use appropriate human cell lines known to express NRP2 (e.g., vascular or neural cell types) and include appropriate controls.
• For ELISA, consider that the antibody may detect recombinant or native NRP2; titration and optimization under experimental conditions are recommended.
• Store antibody aliquots at -20°C or as recommended; avoid repeated freeze-thaw cycles to maintain activity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a trusted one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers, research laboratories, and institutes from concept to clinic. This anti-NRP2 rabbit monoclonal antibody exemplifies our capability to provide validated monoclonal antibodies, antibody pairs, recombinant proteins, and ancillary reagents for neuroscience, cardiovascular, and infectious disease research. Beyond this product, CamelBio sources rare and specialized raw materials, and offers technical and consulting services to accelerate your assay development and production workflows.