Anti-Neuropilin-1 (NRP1) Rabbit Monoclonal Antibody for WB, ELISA - O14786

Core Product Specifications and Parameters

Biological Background: Neuropilin-1 Function and Localization

• Neuropilin-1 (NRP1) is a multi-functional cell-surface receptor also known as Vascular endothelial cell growth factor 165 receptor, CD304, and BDCA4. Alternative splicing yields isoforms with non-overlapping expression patterns, contributing to its diverse tissue-specific functions.
• It binds class 3 semaphorins (e.g., SEMA3A) and VEGF family members (VEGF165, PLGF-2, VEGFB), mediating chemorepulsion and angiogenesis, respectively. Related references: PMID:10688880, PMID:9288753, PMID:9529250
• NRP1 recognizes a C-end rule (CendR) motif (R/KXXR/K) on ligands, triggering cellular internalization and vascular leakage. Related references: PMID:19805273
• Coexpression with VEGFR2/KDR enhances VEGF165 binding and downstream signaling, promoting endothelial cell chemotaxis and angiogenesis.
• In the developing nervous system, NRP1 guides motor neuron axon projection and cell body migration, including facial motor neuron migration from rhombomere 4 to 6.
• NRP1 localizes to the cell membrane, cytoplasm, and is also found secreted and associated with the mitochondrial membrane, reflecting its multifunctional roles.
• In adult tissues, NRP1 is highly expressed in heart and placenta, moderate in lung, liver, skeletal muscle, kidney and pancreas, and low in brain. Related references: PMID:10688880, PMID:9529250

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 824–923 within the C-terminal domain of human NRP1. This region is conserved in mouse and rat, consistent with the reported cross-reactivity. Verify sequence alignment in your species of interest prior to experiments.
• For Western blot (WB) analysis, a band near 103 kDa is expected for full-length NRP1. Consider using membrane protein extraction protocols and reducing conditions, as NRP1 forms disulfide-linked dimers and may exhibit higher-order structures. Validate specificity with appropriate controls (e.g., peptide competition or knockdown).
• For ELISA, this antibody may be suitable as a capture or detection reagent; titrate conditions using recombinant NRP1 or known positive samples and include negative controls to establish assay sensitivity and dynamic range.
• Additional bands may appear due to isoform heterogeneity, glycosylation, or proteoglycan modifications; confirm band identity using mass spectrometry or isoform-specific reagents if needed.
• Store at -20°C or lower upon arrival and avoid repeated freeze-thaw cycles to maintain performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop platform for IVD raw-material sourcing, supporting diagnostic manufacturers and research institutes from discovery to commercial scale. For NRP1-related projects in angiogenesis, neurobiology, and infectious disease, CamelBio offers validated antibody pairs, recombinant proteins, and ancillary reagents. Our sourcing capabilities extend to rare target raw materials, with technical support to accelerate your assay development and manufacturing timelines.