Anti-Neurokinin 1 Receptor (NK1R) Rabbit Monoclonal Antibody for WB, ELISA - P25103

Core Product Specifications and Parameters

Biological Background: Neurokinin 1 Receptor (NK1R) Function and Localization

• Encoded by the TACR1 gene (also NK1R, TAC1R); alternative splicing generates multiple isoforms.
• Belongs to the class A G protein-coupled receptor (GPCR) family, tachykinin receptor subfamily, and functions as the primary receptor for substance P.
• Additionally binds neurokinin A/substance K and neurokinin B/neuromedin-K with lower affinity (PubMed:15452552). Related references: PMID:15452552
• Affinity rank order: substance P > neurokinin A > neurokinin B (PubMed:1718267). Related references: PMID:1718267
• Agonist binding triggers G protein-coupled signaling primarily via phospholipase C-mediated phosphatidylinositol hydrolysis and adenylate cyclase/cAMP pathways.
• Localizes predominantly at the cell membrane; upon stimulation, internalizes into early endosomes.
• Post-translational modifications include N-linked glycosylation, disulfide bonds, and palmitoylation, which are critical for proper folding and membrane localization.
• Implicated in pain perception, neurogenic inflammation, stress responses, and gastrointestinal motility.

Experimental Guidance and Technical Tips

• The immunogen corresponds to an intracellular region (amino acids 300-407) of the receptor; this epitope is likely to detect both unmodified and post-translationally modified forms.
• For Western blot, enrichment of membrane proteins or use of whole-cell lysates from tissues known to express NK1R (e.g., brain, gut) is recommended; the predicted molecular weight is 46 kDa, but glycosylation may lead to a higher apparent molecular weight.
• In ELISA, the antibody can be used for direct detection of the receptor in solubilized membrane preparations or peptide competition assays.
• Optimize primary antibody concentration and incubation conditions for each experimental system; no standard dilution is provided.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers, laboratories, and research institutes with a comprehensive selection of IVD raw materials, from validated monoclonal and polyclonal antibodies to bulk ancillary reagents. For researchers exploring GPCR-mediated signaling and neurokinin biology, this anti-NK1R antibody is available alongside specialized sourcing services for rare targets and custom assay development. Whatever your diagnostic pipeline demands, CamelBio supports every stage from concept to clinic.