Anti-Neurogranin (NRGN) Rabbit Polyclonal Antibody for WB, ELISA - Q92686

Core Product Specifications and Parameters

Biological Background: Neurogranin Function and Localization

• Neurogranin (NRGN), also known as RC3, is a postsynaptic protein primarily expressed in the brain, particularly in the cerebral cortex, hippocampus, and striatum.
• It acts as a "third messenger" substrate of protein kinase C (PKC), playing a crucial role in synaptic development, plasticity, and remodeling.
• NRGN binds calmodulin (CaM) in the absence of calcium, regulating CaM availability and thereby modulating calcium/calmodulin-dependent signaling pathways.
• Phosphorylation by PKC at serine 36 (Ser36) disrupts calmodulin binding, linking synaptic activity to downstream signaling events.
• In Alzheimer disease, neurogranin expression is reduced in dendritic compartments, and it has been investigated as a potential cerebrospinal fluid biomarker for synaptic degeneration.
• Post-translational modifications include acetylation, citrullination, and methylation, which may fine-tune its function and stability.

Experimental Guidance and Technical Tips

• For Western blotting, expect a band around 8 kDa; ensure proper gel formulation and transfer conditions for low molecular weight proteins.
• The immunogen covers the full-length human NRGN (aa 1-78), so the antibody likely recognizes total NRGN; phosphorylation-specific detection would require site-specific antibodies.
• Cross-reactivity with mouse and rat is confirmed, making it suitable for rodent models; always validate in the relevant model system.
• For ELISA, consider using recombinant NRGN or brain tissue lysates as coating antigen; optimize dilution based on your assay conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, including validated antibodies, recombinant proteins, and ancillary reagents. For synaptic biology and neurodegenerative disease research, we offer optimized polyclonal antibodies like this anti-NRGN, along with matched antibody pairs and bulk production options. Whether you need rare target sourcing or custom development, CamelBio bridges your concept to clinic.