Anti-Neurogenin 2 (NGN2) Monoclonal Antibody for WB, ELISA - Q9H2A3

Core Product Specifications and Parameters

Biological Background: Neurogenin 2 Function and Localization

• Neurogenin 2, encoded by the NEUROG2 gene, is a class A basic helix-loop-helix (bHLH) transcription factor, also known as bHLHa8 and protein atonal homolog 4.
• It functions as a transcriptional regulator critical for neuronal differentiation, activating gene expression by binding to the E-box motif (5'-CANNTG-3').
• As a proneural protein, Neurogenin 2 drives neuronal fate specification during development and is a key developmental protein.
• The protein localizes exclusively to the nucleus, consistent with its role as a DNA-binding transcription factor.
• It contains a basic helix-loop-helix domain, typical of the bHLH family, which mediates DNA binding and dimerization.
• UniProt keywords include Activator, Developmental protein, Differentiation, DNA-binding, Neurogenesis, Nucleus, and Transcription regulation, highlighting its central role in gene expression control.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 50–150, which includes part of the functional bHLH domain. This may allow detection of both full-length and truncated forms containing this region.
• For Western blotting, expect a band at approximately 29 kDa (predicted 28.6 kDa) and validate expression in neuronal cell lines or tissues.
• The peptide-specific nature of the immunogen makes the antibody suitable for direct antigen detection in ELISA; consider optimizing coating conditions and detection antibodies.
• Cross-reactivity is reported for human, mouse, and rat; confirm reactivity with your specific sample system, as sequence homology in the immunogen region may vary.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material partner, offering this validated Neurogenin 2 monoclonal antibody for neuroscience and developmental biology applications. Beyond single antibodies, we provide matched antibody pairs, bulk ancillary reagents, and customized sourcing for rare targets—streamlining your path from discovery to diagnostic development. Leverage our supply chain expertise to secure all your immunoassay and molecular biology needs efficiently.