Anti-NEUROG3 Rabbit Polyclonal Antibody for WB, ELISA - Q9Y4Z2

Core Product Specifications and Parameters

Biological Background: Neurogenin-3 Function and Localization

• Neurogenin-3 (NEUROG3) is a basic helix-loop-helix (bHLH) transcription factor encoded by the NEUROG3 gene, also known as ATOH5, BHLHA7, or NGN3.
• It functions as a transcriptional regulator that controls enteroendocrine cell differentiation and, in cooperation with NKX2-2, initiates the transcriptional activation of NEUROD1. Related references: PMID:21378176 PMID:16855267
• NEUROG3 is involved in neurogenesis and is required for the specification of a common precursor of the four pancreatic endocrine cell types (alpha, beta, delta, and PP cells).
• The protein localizes to the nucleus, consistent with its role as a DNA-binding transcriptional activator.
• Keywords associated with NEUROG3 include developmental protein, diabetes mellitus, and disease variant, underscoring its importance in endocrine development and metabolic disease.
• It belongs to the class A bHLH protein family and is recognized as a key regulator in cell fate determination within the neuroendocrine system.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1-100 of human NEUROG3, targeting the N-terminal region. This design may enable recognition of full-length endogenous protein in relevant sample systems.
• For Western blot (WB), the predicted molecular weight of NEUROG3 is approximately 23 kDa. Validate the antibody in your specific cell or tissue lysate, and consider using positive controls known to express NEUROG3.
• When performing ELISA, standard indirect or sandwich ELISA protocols can be applied. Optimize coating antigen and antibody dilutions based on your assay setup.
• As a polyclonal antibody, batch-to-batch variability may exist; inclusion of appropriate internal controls is recommended for reproducible results.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, supporting researchers and manufacturers working on neurogenin-3 and endocrine development. We offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets to streamline your assay development from concept to clinic. Our product portfolio is designed to meet the rigorous demands of diagnostic and life science applications.