Anti-NEUROG3 Polyclonal Antibody for WB, IHC-P, ELISA - Q9Y4Z2

Core Product Specifications and Parameters

Biological Background: NEUROG3 Function and Localization

• NEUROG3 (Neurogenin-3) is a class A basic helix-loop-helix (bHLH) transcription factor, also known as ATOH5 or NGN3, encoded by the NEUROG3 gene.
• Acts as a transcriptional regulator involved in the control of enteroendocrine cell differentiation. Related references: PMID:21378176
• Together with NKX2-2, initiates transcriptional activation of NEUROD1, a key step in endocrine lineage commitment. Related references: PMID:16855267, PMID:21378176
• Required for the specification of a common precursor of the four pancreatic endocrine cell types (by similarity).
• Involved in neurogenesis, highlighting its role in neuronal development.
• Predominantly localizes to the nucleus, consistent with its function as a DNA-binding transcription factor.
• UniProt keywords indicate association with diabetes mellitus and disease variants, reflecting its critical role in pancreatic function.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal 100 amino acids of human NEUROG3. Consider validating reactivity in your target species, as cross-reactivity is stated for human, mouse, and rat.
• For Western blotting, the predicted molecular weight is ~23 kDa. Optimize gel percentage (e.g., 12-15% SDS-PAGE) and transfer conditions for low molecular weight proteins.
• In IHC-P, antigen retrieval may be required; test citrate or EDTA buffer, and include appropriate positive control tissues such as pancreas or intestine.
• For ELISA, this polyclonal antibody can potentially serve as a detection reagent. Pair with a monoclonal capture antibody if developing a sandwich assay; validate performance using recombinant NEUROG3 or cell lysates.
• Always include proper negative controls (e.g., knockout cell lines or tissues) to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—from concept to clinic. This NEUROG3 antibody, targeting a key regulator of endocrine and neuronal development, exemplifies our capability to supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target raw-material sourcing. Partner with CamelBio to streamline your procurement and accelerate your diagnostic development.