Anti-Neurofilament M Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P07197

Core Product Specifications and Parameters

Biological Background: Neurofilament M Function and Localization

• Neurofilament medium polypeptide (NFM, NEF3, 160 kDa neurofilament protein) is a key intermediate filament protein of neurons.
• It assembles with NEFL (light) and NEFH (heavy) to form neurofilaments, which are essential for maintaining axonal caliber and structural integrity.
• NFM may also interact with other neuronal intermediate filament proteins, such as peripherin (PRPH) and internexin (INA), to create extensive filamentous networks.
• Subcellularly, NFM localizes to the cytoplasm, cytoskeleton, axons (cell projections), and perikarya.
• Post-translational modifications, including phosphorylation, acetylation, and methylation, regulate NFM filament dynamics and function.
• Alternative splicing of the NEFM gene yields multiple isoforms, contributing to functional diversity in different neuronal contexts.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 100-260 of human NFM, a central region likely to be conserved, enabling detection of endogenous protein across human, mouse, and rat samples.
• For Western blotting (WB), the expected band is approximately 102 kDa; however, due to post-translational modifications and isoforms, multiple bands or slight shifts may be observed.
• In immunohistochemistry (IHC-P) and immunocytochemistry (IF/ICC), fixation and permeabilization conditions should be optimized as NFM is a cytoskeletal protein; consider using methods that preserve filament structures.
• For ELISA, an appropriate coating antigen and detection system should be validated; the antibody may be used for capture or detection with proper pairing.
• As a rabbit polyclonal antibody, it offers high avidity and recognizes multiple epitopes, which can be advantageous for robust detection in diverse applications.
• Confirm specificity by including appropriate controls (e.g., knockout cell lines or tissues) and by validating against the immunogen if possible.

CamelBio: Your One-Stop Sourcing Bridge

As your comprehensive IVD raw-material partner, CamelBio supports your neurofilament research and neuronal biomarker programs with high-quality antibodies like this anti-NFM polyclonal antibody, along with matched pairs, bulk ancillary reagents, and custom sourcing for rare targets—streamlining your path from discovery to diagnostic deployment.