Anti-Neurofilament L Rabbit Polyclonal Antibody for WB, IF-P, IHC-P, ELISA - P07196

Core Product Specifications and Parameters

Biological Background: Neurofilament Light Polypeptide Function and Localization

• Neurofilament light polypeptide (NEFL), also known as 68 kDa neurofilament protein or neurofilament triplet L protein, is an intermediate filament protein encoded by the NEFL gene (Gene ID: 4747).
• NEFL, along with NEFM and NEFH, forms the core neurofilament triplet that is essential for maintaining neuronal caliber and axonal structure.
• It may also interact with other neuronal intermediate filament proteins such as peripherin (PRPH) and alpha-internexin (INA) to assemble filamentous networks in neurons.
• NEFL is primarily localized to the axon cytoplasm and cytoskeleton, specifically within cell projections, consistent with its role in neuronal architecture.
• The protein undergoes various post-translational modifications including acetylation, phosphorylation, methylation, and ubiquitination, which regulate its assembly and function.
• Mutations in NEFL are associated with Charcot-Marie-Tooth disease (CMT) types 1F, 2E, and Digenic, highlighting its clinical relevance in neurodegeneration.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody is a recombinant protein spanning amino acids 60-250 of human NEFL; consider its potential to recognize epitopes within the rod domain important for filament assembly.
• For Western blotting, a band around the expected 62 kDa should be observed; validate detection in neuronal cell lines or brain tissue lysates.
• Immunofluorescence (IF-P) and immunohistochemistry (IHC-P) can be performed to visualize NEFL distribution in axons and neuronal projections; include appropriate permeabilization and blocking steps.
• Cross-reactivity with mouse and rat orthologs enables testing in rodent models; however, perform a species-specific validation if precise ortholog detection is critical.
• ELISA applications may require titration of the antibody; ensure proper coating conditions and use a validated antigen standard.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines your journey from concept to clinic by offering a comprehensive portfolio of IVD raw materials, including validated antibody pairs, monoclonal and polyclonal antibodies, and bulk ancillary reagents. For neurofilament research and neuronal cytoskeleton studies, CamelBio provides specialized sourcing support for rare targets and custom antibody development. Partner with us to accelerate your diagnostic and research projects with reliable, high-quality raw materials.