Anti-Neurofilament L Monoclonal Antibody for WB, IF, IHC, ELISA - P07196

Core Product Specifications and Parameters

Biological Background: Neurofilament Light Polypeptide Function and Localization

• Neurofilament light polypeptide (NEFL) is a member of the intermediate filament family and is one of the three major neurofilament proteins that also include NEFM and NEFH, which together maintain neuronal caliber and axonal structure.
• NEFL cooperates with other neuronal intermediate filament proteins such as peripherin (PRPH) and alpha-internexin (INA) to form the filamentous networks essential for neuronal integrity.
• The protein is predominantly expressed in axons, localizing to the cytoplasm and cytoskeleton of neuronal cells, and plays a critical role in the radial growth of axons.
• Post-translational modifications include acetylation, glycation, methylation, phosphorylation, and ubiquitination, which regulate filament assembly, stability, and interactions with other cytoskeletal components.
• Mutations in the NEFL gene are linked to neurodegenerative disorders, including Charcot-Marie-Tooth disease types 1F and 2E, highlighting its role in axonal maintenance and susceptibility to neuropathy.
• The protein has a molecular weight of approximately 61.5 kDa (calculated from sequence) and is often detected as a 68 kDa band by SDS-PAGE due to post-translational modifications.
• NEFL, together with other neurofilament subunits, serves as a biomarker for neuronal damage in conditions such as multiple sclerosis, Alzheimer’s disease, and traumatic brain injury.

Experimental Guidance and Technical Tips

• Since the immunogen corresponds to amino acids 60-250, a central region of the human NEFL protein, the antibody likely recognizes a conserved epitope; consider sequence alignment when using it for non-human species to confirm reactivity.
• For Western blot, a band near 68 kDa may be observed; validate by comparing with expected molecular weight and using appropriate loading controls such as β-actin or GAPDH.
• In immunofluorescence, optimal fixation and permeabilization conditions should be tested; paraformaldehyde fixation followed by Triton X-100 permeabilization is commonly used for neurofilament staining.
• For immunohistochemistry, antigen retrieval methods (e.g., heat-mediated citrate buffer) may enhance detection; include positive and negative tissue controls.
• When using the antibody in ELISA, ensure the coating antigen matches the immunogen region to avoid cross-reactivity with other neurofilament subunits.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents, supporting diagnostic manufacturers from concept to clinic. For research involving neuronal biomarkers and neurodegenerative disease diagnostics, this Neurofilament L rabbit monoclonal antibody is part of our reliable neurofilament panel, and we can additionally source rare target raw materials upon request. Partner with CamelBio to streamline your supply chain with expert technical consultation and seamless procurement.