Anti-Neurofilament H Polyclonal Antibody for WB, IP, IF, ELISA - P12036

Core Product Specifications and Parameters

Biological Background: Neurofilament H Function and Localization

• Neurofilament H (NFH) is the heavy polypeptide of the neurofilament triplet, along with light (NEFL) and medium (NEFM) subunits.
• It plays a critical role in maintaining axonal caliber, a function not fully compensated by the smaller neurofilament proteins.
• NEFH may cooperate with peripherin (PRPH) and internexin (INA) to form neuronal filamentous networks.
• Localizes to the cytoplasm, cytoskeleton, and specifically to axons in neurons.
• Subject to post-translational phosphorylation, which regulates filament assembly and interactions.
• Genetic variants in NEFH are associated with Charcot-Marie-Tooth disease type 2CC and amyotrophic lateral sclerosis.
• Keywords highlight its involvement in neurodegeneration and neuropathy.

Experimental Guidance and Technical Tips

• The immunogen spans the C-terminal tail region (amino acids 873–1020), which contains multiple phosphorylation sites; consider this when assessing potential phospho-specific reactivity.
• For western blot, expect a band around 112 kDa; validate with appropriate positive and negative controls in your sample system.
• Suitable for immunofluorescence on cultured cells (IF/ICC) and paraffin-embedded sections (IF-P); optimize fixation and permeabilization conditions for best results.
• For immunoprecipitation, test different antibody-to-lysate ratios to achieve efficient capture of the target protein.
• In ELISA, the antibody can be used for antigen detection; confirm specificity with recombinant protein or cell lysates.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for IVD raw materials, offering a streamlined sourcing experience from discovery to commercialization. This anti-Neurofilament H polyclonal antibody supports research in neurobiology and diagnostic development, and we can supply it together with validated antibody pairs, bulk reagents, and ancillary materials. Leverage our global supply network to secure rare-target raw materials and accelerate your project timelines.