Anti-Neurofilament H Monoclonal Antibody for WB, IP, ELISA - P12036

Core Product Specifications and Parameters

Biological Background: Neurofilament H (NFH) Function and Localization

• Neurofilament H (NFH), also known as 200 kDa neurofilament protein or neurofilament triplet H protein, is a heavy polypeptide component of neuronal intermediate filaments.
• NFH is essential for the maintenance of neuronal caliber and has a critical role in mature axons that is not compensated by the smaller neurofilament proteins NEFL and NEFM.
• It may cooperate with other neuronal intermediate filament proteins such as peripherin (PRPH) and internexin (INA) to form filamentous networks in neurons (By similarity).
• The protein localizes to the cytoplasm, particularly within the cytoskeleton of axons, where it contributes to structural integrity and axonal transport.
• NFH undergoes extensive post-translational phosphorylation, which is thought to regulate filament assembly and interactions with other cytoskeletal elements.
• Alternative splicing of the NEFH gene produces multiple isoforms, potentially modulating its function.
• Genetic variants in NEFH are associated with neurodegenerative diseases, including amyotrophic lateral sclerosis (ALS) and Charcot-Marie-Tooth disease type 2CC (CMT2CC), highlighting its clinical relevance.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 550–650 of human Neurofilament H, a region that may be conserved across species (based on cross-reactivity data), suggesting a robust antigenic epitope.
• For Western Blot (WB), expect an approximately 112 kDa band; optimization of sample preparation and detection conditions is recommended using appropriate neuronal cell or tissue lysates.
• In immunoprecipitation (IP) applications, pre-clearing and antibody titration may improve specificity; subsequent WB detection can confirm the target.
• ELISA can be employed for quantitative detection; use purified protein or peptide as a control and validate in the relevant biological matrix.
• Due to the potential for post-translational modifications (e.g., phosphorylation), consider using phosphatase inhibitors during sample preparation to preserve the endogenous state.
• Cross-reactivity with mouse and rat Neurofilament H is indicated, enabling use in rodent models; nonetheless, sequence alignment of the epitope region in target species is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects you with validated Neurofilament H antibodies and a comprehensive portfolio of IVD raw materials for neuroscience research and diagnostic development. Whether you need optimized monoclonal antibodies, bulk ancillary reagents, or assistance sourcing rare targets, our one-stop platform streamlines your supply chain from concept to clinic. Partner with us to accelerate your neurodegenerative disease assay development and production.