Anti-NEUROD6 Polyclonal Antibody for WB, ELISA - Q96NK8

Core Product Specifications and Parameters

Biological Background: NEUROD6 Function and Localization

• Neurogenic differentiation factor 6 (NEUROD6), also known as Class A basic helix-loop-helix protein 2 (bHLHa2) and Protein atonal homolog 2, is encoded by the NEUROD6 (ATOH2, BHLHA2) gene in humans.
• NEUROD6 functions as a transcriptional activator by binding to E box sequences as a heterodimer with TCF3/E47, playing a crucial role in the development and maintenance of the mammalian nervous system.
• The protein contains a basic helix-loop-helix (bHLH) DNA-binding domain characteristic of the NEUROD family of neuronal determination factors.
• NEUROD6 localizes to the nucleus, consistent with its role as a transcription factor regulating gene expression programs during neuronal differentiation.
• It transactivates its own promoter via a positive auto‑regulatory feedback loop, sustaining its expression in committed neuronal lineages.
• The protein is involved in neurogenesis and cell differentiation, acting as a developmental protein that drives the acquisition of neuronal identity.
• Proteomics studies have identified NEUROD6 as part of the reference proteome, underscoring its conserved role in nervous system development.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fragment spanning amino acids 188‑337, which includes the bHLH domain; therefore the antibody primarily recognizes the DNA‑binding region of NEUROD6.
• For Western blot, the predicted molecular weight is ~39 kDa; consider using nuclear extracts or whole‑cell lysates from neuronal tissues or cell lines, as NEUROD6 is a low‑abundance transcription factor.
• Cross‑reactivity with mouse and rat is reported; validate the antibody in the relevant species by comparing the observed band size with the predicted molecular weight.
• ELISA experiments should include appropriate controls, such as coating with the immunogen fragment and testing serial dilutions to assess binding specificity.
• Due to its nuclear localization and role in transcription, denaturing and reducing conditions are typically suitable for SDS‑PAGE, but epitope accessibility may be improved by mild fixation in immunoassays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with reliable access to IVD raw materials, including validated polyclonal antibodies like this NEUROD6 antibody. From transcription factors to neuronal markers, CamelBio offers a broad portfolio of antibodies, antibody pairs, and ancillary reagents to support neurodevelopmental research and assay development. For specialized needs—such as rare target antigens and custom immunogen design—CamelBio serves as your procurement partner, streamlining supply from concept to clinic.