Anti-NEUROD1 Rabbit Polyclonal Antibody for IHC-P, ELISA - Q13562

Core Product Specifications and Parameters

Biological Background: NEUROD1 Function and Localization

• NEUROD1 (Neurogenic differentiation factor 1), also known as BETA2, BHF-1, or bHLHa3, is a class A basic helix-loop-helix transcription factor encoded by the NEUROD1 gene (Gene ID: 4760).
• It functions as a transcriptional activator by binding to E box-containing consensus sequences (5'-CANNTG-3') in target gene promoters, associating with coactivator complexes such as p300/CBP to drive transcription of genes like secretin and CDKN1A.
• NEUROD1 is pivotal in neurogenesis, directing differentiation of early retinal ganglion cells, inner ear sensory neurons, cerebellar and hippocampal granule cells, and amacrine cells (in cooperation with PAX6 or SIX3).
• Beyond the nervous system, it is essential for endocrine cell development, including pancreatic islet β-cells and enteroendocrine cells of the small intestine, linking it to pathways of diabetes mellitus (MODY6).
• The protein localizes to both the cytoplasm and nucleus, consistent with its regulated nuclear import for transcriptional activity.
• Post-translational modifications include phosphorylation, and the protein is implicated in dendrite morphogenesis and maintenance in the cerebellar cortex.
• Keywords from UniProt: Activator, Cytoplasm, Nucleus, Developmental protein, Differentiation, DNA-binding, Neurogenesis, Phosphoprotein, Transcription regulation, and Disease variant (Diabetes mellitus).

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein covering the full-length human NEUROD1 (aa 1-356). This design may generate polyclonal antibodies recognizing multiple epitopes, offering robust detection in IHC-P and ELISA.
• For IHC-P, consider optimizing antigen retrieval and antibody dilution in relevant tissues (e.g., brain, pancreas). Expect nuclear staining with possible cytoplasmic background, and validate with appropriate controls.
• In ELISA, the antibody can be used as a capture or detection reagent; titration and cross-reactivity testing against related bHLH family members is recommended.
• Given the cross-reactivity with mouse, this antibody is suitable for studies in mouse models; however, independent validation in your experimental system is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop partner for IVD raw-material sourcing, offering a comprehensive portfolio that includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and customized solutions for rare targets. For researchers and manufacturers working on neurodevelopment or diabetes, our NEUROD1 antibody exemplifies the high-quality tools we provide to accelerate assay development and streamline procurement. Let CamelBio bridge your raw-material needs from discovery to clinic.