Anti-Neuraminidase/NEU1 Rabbit Monoclonal Antibody for WB, IF-P, ELISA - Q99519

Core Product Specifications and Parameters

Biological Background: Neuraminidase/NEU1 Function and Localization

• Encoded by the NEU1 gene, sialidase-1 (neuraminidase-1) is a 45 kDa lysosomal exo-α-sialidase that catalyzes the removal of terminal sialic acid residues from glycoproteins and glycolipids. Its enzymatic activity requires assembly into a multienzyme complex. Related references: PMID:14695530, PMID:25153125, PMID:37205763
• Sialidase-1 exhibits substrate preference for α2-3 and α2-6 sialyl linkages, contributing to glycoconjugate catabolism and lipid degradation.
• NEU1 localizes predominantly to the lysosomal lumen and membrane; it can also traffic to the cell membrane.
• Expression is highest in pancreas, followed by skeletal muscle, kidney, placenta, heart, lung, and liver; weak in brain.
• Post-translational modifications include disulfide bond formation, N-linked glycosylation, and phosphorylation.
• Mutations in NEU1 cause sialidosis, a lysosomal storage disorder characterized by lysosomal accumulation of sialylated substrates.

Experimental Guidance and Technical Tips

• The immunogen spans the core catalytic domain (aa 48-415) of human NEU1 and may recognize both precursor and mature protein forms.
• For Western blot, a predominant band near 45 kDa is expected in human and mouse lysates; consider including reducing agents in sample buffer.
• In immunofluorescence, a punctate perinuclear pattern consistent with lysosomes is anticipated; co-staining with lysosomal markers is advised.
• ELISA utility should be empirically validated using recombinant NEU1 protein or appropriate cell/tissue extracts as coating material.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research labs with a comprehensive portfolio of IVD raw materials, including validated antibody pairs and monoclonal/polyclonal antibodies like this Neuraminidase/NEU1 clone. Our supply scope covers bulk reagents, ancillary materials, and rare target sourcing—streamlining procurement for lysosomal enzyme studies and metabolic assay development. Partner with CamelBio to accelerate your project from concept to clinic.