Anti-NeuN Mouse Monoclonal Antibody for WB, IF, IHC, ELISA - A6NFN3

Core Product Specifications and Parameters

Biological Background: NeuN Function and Localization

• NeuN is encoded by the RBFOX3 gene and is a member of the FOX family of RNA-binding proteins, functioning as a pre-mRNA alternative splicing regulator.
• It specifically regulates the alternative splicing of RBFOX2 to promote nonsense-mediated decay (NMD) of target transcripts.
• NeuN is predominantly expressed in neuronal nuclei, serving as a widely used post-mitotic neuron marker in neuroscience research.
• The protein localizes to both the nucleus and cytoplasm, consistent with its roles in mRNA processing and transport.
• Post-translational modifications include methylation, as indicated by proteomics identification and reference proteome annotations.
• Keywords associated with NeuN include alternative splicing, mRNA processing, mRNA splicing, nucleus, and cytoplasm, highlighting its key biological roles.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant full-length or fragment of human NeuN; consider optimizing antibody dilution for your specific sample type and detection method.
• For western blot (WB), the predicted molecular weight is ~34 kDa; validate the band with appropriate positive and negative controls.
• In immunohistochemistry (IHC), NeuN shows strong nuclear staining in neurons; ensure proper antigen retrieval and tissue fixation for paraffin-embedded sections.
• For immunofluorescence (IF), both frozen (IF-F) and paraffin (IF-P) samples can be used; test with appropriate neuronal cell lines or brain tissue.
• Cross-reactivity with human, mouse, and rat has been reported; validate specificity in your species of interest before large-scale experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers and research laboratories with validated monoclonal antibodies like this anti-NeuN clone. We provide bulk ancillary reagents and can assist in sourcing rare targets to bridge your neuroscience research needs. Contact us to explore custom antibody solutions and technical support for your diagnostic development pipeline.