Anti-NeuN Monoclonal Antibody for WB, IHC, IF - A6NFN3

Core Product Specifications and Parameters

Biological Background: NeuN Function and Localization

• Encoded by the RBFOX3 gene, NeuN (RNA binding protein fox-1 homolog 3) is also known as Fox-1 homolog C or neuronal nuclei antigen.
• Functions as a pre-mRNA alternative splicing regulator; specifically modulates RBFOX2 splicing to enhance nonsense-mediated decay target production.
• Localizes to both the nucleus and cytoplasm, with nuclear presence consistent with its role in splicing regulation.
• Classified as an RNA-binding protein, suggesting the presence of RNA recognition motifs critical for mRNA processing.
• Post-translational modifications include methylation, which may influence its regulatory activity or subcellular distribution.
• Recognized as a reference proteome member in UniProt, ensuring high-quality functional annotation.
• Widely utilized as a neuronal nuclei marker based on its identification as the NeuN antigen.

Experimental Guidance and Technical Tips

• The immunogen corresponds to an N-terminal peptide (aa 1-100); consider peptide blocking controls to confirm detection specificity.
• For Western blot, the predicted molecular weight is 34 kDa; use standard SDS-PAGE conditions appropriate for this size range.
• For immunostaining applications (IF/IHC), nuclear permeabilization or antigen retrieval steps may be required for optimal epitope access.
• Cross-reactivity with human, mouse, and rat makes the antibody suitable for common model organisms in neuroscience research.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports neuroscience diagnostic development with validated antibodies like this anti-NeuN monoclonal antibody. Our integrated supply chain delivers consistent quality and batch-to-batch reproducibility for IVD manufacturers, from monoclonal antibodies and validated pairs to bulk ancillary reagents. We offer a comprehensive sourcing solution for rare and routine raw materials, streamlining your path from concept to clinic.