Anti-NeuN (FOX-3) Rabbit Polyclonal Antibody for WB, IF-P, IHC-P, ELISA - A6NFN3

Core Product Specifications and Parameters

Biological Background: NeuN Function and Localization

• RBFOX3, commonly referred to as NeuN (Neuronal nuclei antigen), Fox-1 homolog C, or FOX-3, is an RNA-binding protein encoded by the RBFOX3 gene (GeneID: 146713) in humans.
• As a pre-mRNA splicing regulator, it controls alternative splicing of transcripts such as RBFOX2, directing isoforms towards nonsense-mediated decay (NMD).
• Subcellularly, RBFOX3 localizes to both the nucleus and cytoplasm, consistent with its roles in RNA processing and transport.
• Post-translational modifications include methylation, although the functional impact remains to be fully elucidated.
• UniProt keywords associated with RBFOX3 include alternative splicing, mRNA processing, mRNA splicing, RNA-binding, nucleus, cytoplasm, and methylation.
• The protein's alias, NeuN, underscores its utility as a definitive marker for post-mitotic neurons in developmental and disease studies.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal region (aa 1-220), which may recognize multiple isoforms or conserved domains. Validate antibody specificity in knockout or knockdown models.
• For Western blot (WB), an expected band around 34 kDa should be observed. Use appropriate positive controls such as human, mouse, or rat brain lysates.
• In immunofluorescence (IF-P) and immunohistochemistry (IHC-P), nuclear and cytoplasmic staining may be detected. Antigen retrieval may be necessary for paraffin-embedded tissues.
• ELISA and other applications may require optimization of coating and detection conditions.
• Cross-reactivity with human, mouse, and rat has been demonstrated, but additional validation is advised for other species.
• As a polyclonal antibody, lot-to-lot consistency should be considered for critical quantitative assays.
• This antibody is suitable for detecting NeuN in neuronal lineages; co-staining with other neuronal markers can enhance verification.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive access to IVD raw materials, including validated antibodies for neurological targets such as NeuN. Our platform supports diagnostic manufacturers and researchers with optimized polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets, streamlining the path from concept to clinic.