Anti-NEU3 Polyclonal Antibody for WB, IF/ICC, ELISA - Q9UQ49

Core Product Specifications and Parameters

Biological Background: NEU3 Function and Localization

• NEU3, also known as sialidase-3 or ganglioside sialidase, is encoded by the NEU3 gene and belongs to the neuraminidase family of exo-alpha-sialidases.
• It catalyzes the removal of terminal sialic acid residues from gangliosides, glycoproteins, and oligosaccharides, with high catalytic efficiency for gangliosides such as GD1a, GM3, and GD3. Related references: PMID:10405317 PMID:10861246 PMID:11298736
• NEU3 modulates transmembrane signaling by altering ganglioside composition in the lipid bilayer and by directly interacting with receptors like EGFR, leading to desialylation and activation of downstream signaling in proliferating cells. Related references: PMID:17334392 PMID:25922362
• The protein contributes to clathrin-mediated endocytosis by regulating the sorting of endocytosed receptors to early and recycling endosomes. Related references: PMID:26251452
• Subcellularly, NEU3 localizes to the plasma membrane, caveolae, and membranes of endosomes (early and recycling) and lysosomes.
• Tissue expression is highest in skeletal muscle, testis, adrenal gland, thymus, pancreas, liver, and heart, with lower levels in kidney, placenta, brain, and lung.
• Post-translational modifications include phosphorylation, and the gene undergoes alternative splicing, giving rise to multiple isoforms.
• The canonical isoform has a predicted molecular weight of approximately 48 kDa.

Experimental Guidance and Technical Tips

• For Western blot, a band near 48 kDa corresponding to NEU3 is expected; validate with appropriate positive and negative controls, such as cell lines with known expression levels.
• In immunofluorescence/immunocytochemistry, permeabilization may be required to access intracellular pools, given NEU3's association with multiple membrane compartments.
• Antibody reactivity has been confirmed against human and mouse NEU3; cross-reactivity with other species should be verified experimentally before use.
• For ELISA applications, titrate antibody to obtain optimal signal-to-noise ratio with the recombinant or native protein of interest.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic developers and researchers with streamlined access to premium IVD raw materials, including validated antibodies like this anti-NEU3 polyclonal antibody. Our portfolio supports ganglioside metabolism and cell signaling research with bulk reagents, optimized antibody pairs, and custom sourcing for rare targets. Partner with us to accelerate your assay development from concept to clinic.