Anti-NEU2 Rabbit Polyclonal Antibody for WB, ELISA - Q9Y3R4

Core Product Specifications and Parameters

Biological Background: NEU2 Function and Localization

• Sialidase-2 (NEU2, also known as cytosolic sialidase, N-acetyl-alpha-neuraminidase 2) is a glycosidase that hydrolyzes terminal sialic acid residues, participating in glycolipid, glycoprotein, and oligosaccharide degradation. Related references: PMID:14613940 PMID:22228546
• Displays substrate preference for alpha-(2->3)-sialylated gangliosides (GD1a, GT1b) over alpha-(2->8)-linked sialic acids; active on monomeric GM1 but not micellar form; cleaves alpha-(2->3)-sialyllactose but not alpha-(2->6)-sialyllactose.
• Localizes to the cytoplasm and cytosol.
• Expressed in skeletal muscle, fetal liver, and embryonic carcinoma cells (NT2-D1).
• Involved in lipid degradation and carbohydrate metabolism; contains repeat elements as indicated by UniProt keywords.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 171–380 of human NEU2, targeting the C-terminal region; this design may facilitate detection of both native and denatured forms.
• For Western blot, consider using lysates from skeletal muscle or NT2-D1 embryonic carcinoma cells, where NEU2 is reported to be expressed.
• ELISA performance should be validated using recombinant NEU2 protein or relevant sialylated glycoprotein substrates under optimized conditions.
• As a polyclonal antibody, lot-to-lot consistency and cross-reactivity should be verified in the intended sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a global IVD raw-material supplier offering one-stop procurement, technical services, and consulting for diagnostic manufacturers and research labs, supporting the full development path from concept to clinical application. This NEU2 antibody and related reagents assist in sialidase-focused glycolipid metabolism studies, with options for validated antibody pairs, bulk ancillary reagents, and custom rare-target raw-material sourcing. Reach out to streamline your assay development with reliable supply and expert support.