Anti-NET1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q7Z628

Core Product Specifications and Parameters

Biological Background: NET1 Function and Localization

• NET1 (Neuroepithelial cell-transforming gene 1) is a proto-oncogene also known as ARHGEF8 and p65 Net1. It functions as a guanine nucleotide exchange factor (GEF) specifically activating RhoA GTPase.
• The protein is involved in cytoskeletal reorganization and signal transduction pathways, including potential activation of the SAPK/JNK pathway.
• NET1 stimulates genotoxic stress-induced RHOB activity in breast cancer cells, contributing to cell death.
• It localizes to both the cytoplasm and nucleus, suggesting shuttling roles.
• The protein is subject to post-translational modifications such as acetylation and phosphorylation, as indicated by proteomics identification.
• Widely expressed in human tissues, with alternative splicing generating multiple transcript variants.
• Structural studies (3D-structure) have been reported, providing insights into its domain organization and regulation.
• As a proto-oncogene, NET1 is of significant interest in cancer research, particularly in pathways governing cell proliferation and survival.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1-300 of human NET1 (NP_001040625.1), encompassing the N-terminal region. Consider verifying binding to endogenous NET1 at the expected molecular weight (~68 kDa) in your sample system.
• For western blotting, perform a titration of primary antibody and include appropriate positive controls (e.g., lysates from cell lines with known NET1 expression) and negative controls.
• In immunofluorescence/immunocytochemistry, evaluate subcellular localization signals; both cytoplasmic and nuclear staining may be observed.
• For ELISA, validate sensitivity and specificity using recombinant NET1 protein or peptide-based competition assays.
• Since the antibody cross-reacts with human and mouse NET1, it may be used across these species; however, confirm reactivity in the intended model system before large-scale studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with streamlined access to premium IVD raw materials. From validated antibody pairs and optimized polyclonals like this NET1 antibody to bulk ancillary reagents and rare-target sourcing, we support your entire development pipeline—from concept to clinic. Whether you're advancing oncology biomarker research or building robust detection kits, our one-stop model ensures you receive consistent, scalable supply along with responsive technical consultation.