Anti-Nestin Rabbit Monoclonal Antibody for WB, IF/ICC, IF-P, IHC-P, ELISA - P48681

Core Product Specifications and Parameters

Biological Background: Nestin Function and Localization

• Nestin is a type VI intermediate filament protein essential for brain and eye development.
• It promotes the disassembly of phosphorylated vimentin intermediate filaments during mitosis, facilitating the trafficking and distribution of IF proteins and other cellular factors to daughter cells.
• Nestin is required for survival, renewal, and mitogen-stimulated proliferation of neural progenitor cells.
• Expression is restricted to central nervous system (CNS) stem cells.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitination (isopeptide bond formation).
• Contains a coiled-coil domain, characteristic of intermediate filament proteins.
• Acts as a developmental protein involved in neurogenesis.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1392-1621 of human Nestin (NP_006608.1). Consider this region when assessing epitope accessibility in different applications.
• For Western blot, a band at approximately 177 kDa is expected; use appropriate molecular weight markers and validate with a relevant positive control (e.g., neural stem cell lysates).
• In immunofluorescence (IF/ICC) and immunohistochemistry (IHC), optimize permeabilization and blocking steps to detect filamentous structures. Co-staining with vimentin or other IF proteins may aid localization.
• ELISA applications may require the full-length recombinant protein or specific peptide coating; optimize coating concentration and detection system.
• Verify antibody specificity in your experimental system using knockdown, knockout, or peptide competition assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for diagnostic manufacturers and research labs, offering validated Nestin antibodies and related IVD raw materials. For CNS stem cell and neurodevelopment studies, we can supply optimized antibody pairs, bulk monoclonal antibodies, and ancillary reagents. Our one-stop model also facilitates the procurement of rare-target raw materials and technical consulting, streamlining your transition from concept to clinical application.