Anti-Nestin Polyclonal Antibody for WB - P48681

Core Product Specifications and Parameters

Biological Background: Nestin Function and Localization

• Nestin, encoded by the NES gene, is a 177 kDa class VI intermediate filament protein predominantly expressed in CNS stem cells.
• It is required for brain and eye development; promotes the disassembly of phosphorylated vimentin intermediate filaments during mitosis.
• Plays a role in the trafficking and distribution of intermediate filament proteins and other cellular factors to daughter cells during progenitor cell division.
• Required for survival, renewal, and mitogen-stimulated proliferation of neural progenitor cells.
• Localizes to the cytoplasm as part of the intermediate filament network.
• Contains a central coiled-coil domain characteristic of intermediate filament proteins.
• Post-translational modifications include acetylation, phosphorylation, and isopeptide bonds (Ubl conjugation).
• Associated keywords: developmental protein, neurogenesis, phosphoprotein.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal region (aa 1392–1621) of human Nestin; this design ensures specific recognition of Nestin.
• For western blotting (WB), consider loading 20–30 µg of lysate and validate with appropriate positive/negative controls.
• For immunohistochemistry (IHC-P), antigen retrieval may be required; optimize using known positive tissues such as developing brain or neural stem cells.
• For immunofluorescence (IF/ICC), permeabilization is necessary to access cytoskeletal Nestin.
• Due to cross-reactivity with mouse and rat, validate antibody performance in the relevant model system.
• The polyclonal nature may provide robust signal but consider lot-to-lot consistency.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with comprehensive access to IVD raw materials, including this high-quality anti-Nestin polyclonal antibody for neural stem cell and neurogenesis research. Our portfolio spans validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and rare-target raw-material sourcing, supporting your workflow from concept to clinic.