Anti-Nesprin 1 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q8NF91

Core Product Specifications and Parameters

Biological Background: Nesprin 1 Function and Localization

• Nesprin-1 (Nuclear envelope spectrin repeat protein 1), encoded by the SYNE1 gene, is a multi-isomeric modular protein with a full-length molecular weight of approximately 1011 kDa. Multiple splice variants give rise to tissue-specific isoforms, including a smaller ~112 kDa form.
• As a core component of the LINC (Linker of Nucleoskeleton and Cytoskeleton) complex, Nesprin-1 connects the nuclear lamina to the actin cytoskeleton, facilitating mechanical force transmission across the nuclear envelope and regulating nuclear positioning and movement.
• SYNE1 acts redundantly with its paralog SYNE2 in processes such as nucleus-centrosome attachment and nuclear migration in neural progenitors, often in association with SUN domain proteins and cytoplasmic dynein-dynactin motor complexes.
• Subcellularly, Nesprin-1 localizes predominantly to the outer nuclear membrane but is also found at the nuclear envelope, cytoskeleton, sarcomeres, and Golgi apparatus, reflecting its diverse roles in intracellular architecture.
• The protein contains spectrin repeats and a C-terminal KASH domain, which anchors it to the nuclear envelope. It is subject to phosphorylation and potentially other post-translational modifications, as indicated by proteomics data.
• Nesprin-1 is widely expressed, with highest levels in skeletal and smooth muscle, heart, spleen, and several other tissues. A short isoform (GSRP-56) is predominantly expressed in heart and skeletal muscle.
• Mutations in SYNE1 are linked to Emery-Dreifuss muscular dystrophy and other disorders, underscoring the protein's critical role in muscle function and nuclear mechanics.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 8220–8410 within the spectrin-repeat region of human Nesprin-1 isoform 2. This sequence is conserved in mouse and rat orthologs, supporting cross-reactivity.
• For Western blot detection, note that full-length Nesprin-1 (~1011 kDa) requires specialized high-molecular-weight protein transfer protocols. A more readily detectable band near 112 kDa may represent smaller isoforms or cleavage products.
• In immunofluorescence applications, Nesprin-1 typically exhibits a nuclear rim and cytoplasmic filamentous staining pattern; appropriate fixation (e.g., 4% PFA) and permeabilization are recommended.
• When used in immunohistochemistry on paraffin sections, antigen retrieval may be necessary; validate staining specificity with appropriate positive and negative controls.

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