Anti-Nephrin Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - O60500

Core Product Specifications and Parameters

Biological Background: Nephrin Function and Localization

• Nephrin is a renal glomerulus-specific cell adhesion receptor encoded by the NPHS1 gene, also known as NPHN or CNF. It is a transmembrane protein with immunoglobulin domains, disulfide bonds, and glycosylation sites (UniProt O60500).
• It plays a critical role in the development and function of the kidney glomerular filtration barrier, regulating vascular permeability and anchoring the podocyte slit diaphragm to the actin cytoskeleton.
• Nephrin is specifically expressed in podocytes of kidney glomeruli, consistent with its role in slit diaphragm integrity.
• The protein is localized at the cell membrane and undergoes alternative splicing, producing isoforms with potential functional diversity.
• Post-translational modifications include phosphorylation and glycosylation, which may regulate its activity and interactions.
• By similarity, Nephrin has been implicated in skeletal muscle formation through regulation of myoblast fusion, suggesting roles beyond kidney.
• Disease-associated variants of NPHS1 are linked to congenital nephrotic syndrome, highlighting its clinical significance.
• Keywords associated with Nephrin: cell adhesion, developmental protein, disease variant, glycoprotein, membrane, phosphoprotein, and myogenesis.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (amino acids 1019-1241) of human Nephrin, which is intracellular and may be less glycosylated; this design likely contributes to cross-reactivity with mouse and rat.
• For Western blot, the expected molecular weight is approximately 135 kDa; consider using reducing conditions to resolve the mature glycosylated form.
• In IHC-P, specific staining is expected in glomerular podocytes; optimize antigen retrieval and detection methods for formalin-fixed paraffin-embedded kidney sections.
• For ELISA, pairing with a detection antibody targeting a different epitope is recommended; this monoclonal antibody can be used as capture or detection antibody.
• Validate cross-reactivity in mouse and rat kidney samples, as sequence homology in the immunogen region may affect reactivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-Nephrin rabbit monoclonal antibody is a critical tool for glomerular disease research and diagnostic assay development. CamelBio supports your projects with validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing.