Anti-Nephrin Polyclonal Antibody for WB, IF, IHC, ELISA - Q9QZS7

Core Product Specifications and Parameters

Biological Background: Nephrin Function and Localization

• Nephrin is a renal glomerulus-specific cell adhesion receptor that plays critical roles in kidney development and function.
• It is an essential component of the glomerular filtration barrier, where it regulates vascular permeability and anchors the podocyte slit diaphragm to the actin cytoskeleton.
• Nephrin also participates in skeletal muscle formation by regulating myoblast fusion, indicating extra-renal functions.
• The protein localizes to the cell membrane and is predominantly expressed in kidney podocytes, with notable expression in developing CNS tissues (cerebellum, hindbrain, spinal cord, retina, hypothalamus) and skeletal muscle.
• Multiple isoforms exist through alternative splicing; isoform 1 is the major form in adult kidney, whereas isoform 2 is expressed in the developing brain.
• Nephrin is a type I transmembrane protein with an extracellular region containing immunoglobulin domains, a single transmembrane helix, and a cytoplasmic tail that undergoes phosphorylation, which is critical for signaling and structural interactions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1033-1256 of mouse nephrin (intracellular domain), so the antibody is expected to detect the full-length protein and potentially its cytoplasmic fragments.
• For Western blotting, a specific band at approximately 135-140 kDa should be observed in mouse or rat kidney lysates; note that nephrin may be glycosylated, which can shift the apparent molecular weight.
• In immunofluorescence and immunohistochemistry, expect membranous staining in glomerular podocytes; frozen and paraffin sections are compatible as per the stated applications.
• As a rabbit polyclonal antibody, it is well-suited for co-staining with mouse monoclonal antibodies in multiplex assays; validate cross-reactivity and specificity in your experimental system.
• For ELISA, the antibody can be used as capture or detection reagent; optimization with the appropriate recombinant protein or native samples is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with one-stop access to high-quality IVD raw materials. For nephrin-related projects, we supply validated antibody pairs, bulk polyclonal and monoclonal antibodies, and ancillary reagents to support kidney disease biomarker development and research. Our sourcing capabilities span rare targets and customized raw materials, ensuring your transition from concept to clinic is seamless.