Anti-NELL2 Polyclonal Antibody for WB, ELISA - Q99435

Core Product Specifications and Parameters

Biological Background: NELL2 Function and Localization

• Protein kinase C-binding protein NELL2 (also known as NEL-like protein 2 or Nel-related protein 2) is encoded by the NELL2 gene (alias NRP2).
• NELL2 plays multiple roles in neural tissues, regulating neuronal proliferation, survival, differentiation, polarization, axon guidance, and synaptic functions.
• Through interaction with its receptor ROBO3, NELL2 acts as a repulsive axon guidance cue, contributing to commissural axon guidance to the midline. Related references: PMID:32198364
• NELL2 is required for neuron survival via modulation of MAPK signaling pathways.
• NELL2 is involved in the regulation of hypothalamic GNRH secretion and the control of puberty.
• As an epididymal-secreted protein, NELL2 signals through a ROS1-pathway to regulate epididymal initial segment maturation, sperm maturation, and male fertility.
• Subcellular localization: Secreted protein.
• Contains key structural features including EGF-like domains, calcium-binding, disulfide bonds, and is subject to alternative splicing and glycosylation.

Experimental Guidance and Technical Tips

• Consider using this antibody for Western blot detection of NELL2 (predicted MW ~91 kDa) in human, mouse, and rat samples.
• For ELISA applications, validate the antibody in the appropriate sample matrix and optimize coating antigen concentrations.
• The immunogen corresponds to the N-terminal region (aa 22-300), so the antibody may recognize full-length NELL2 and certain isoforms.
• Cross-reactivity with mouse and rat has been reported; confirm specificity in the target species before large-scale experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with streamlined access to high-quality IVD raw materials like this NELL2 antibody, supporting studies in neurobiology and developmental biology. Beyond antibodies, we offer a comprehensive portfolio including validated antibody pairs, optimized polyclonal/monoclonal antibodies, and bulk ancillary reagents. Our team also assists with rare target raw-material sourcing to accelerate your assay development.