Anti-NELL1 Rabbit Polyclonal Antibody for WB and ELISA - Q92832

Core Product Specifications and Parameters

Biological Background: NELL1 Function and Localization

• NELL1 (Protein kinase C-binding protein NELL1), also known as NEL-like protein 1 or Nel-related protein 1, is encoded by the NELL1 (NRP1) gene.
• It is a multidomain secretory glycoprotein containing EGF-like domains, calcium-binding repeats, and a signal peptide.
• NELL1 plays a role in the control of cell growth and differentiation, specifically promoting osteoblast cell differentiation and terminal mineralization.
• Subcellularly, it localizes to the cytoplasm, nuclear envelope, and is secreted into the extracellular space.
• Post-translational modifications include disulfide bond formation and glycosylation; alternative splicing generates multiple isoforms.
• Associated keywords highlight its structural and functional features: 3D-structure, calcium binding, differentiation, EGF-like domain, glycoprotein, nucleus, secreted, and signal.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 160-420; reactivity to both native and recombinant NELL1 is expected, but validation in your specific assay system is recommended.
• For Western blot, a band at approximately 90 kDa should be detected; optimize loading and antibody dilution using osteoblast or bone-derived positive controls.
• In ELISA, this polyclonal antibody can serve as a capture or detection reagent; consider pairing with a monoclonal antibody for sandwich ELISA development.
• Cross-reactivity with mouse and rat NELL1 is reported, supporting preclinical rodent studies; verify sequence homology to ensure specificity.
• Because NELL1 is secreted, cell culture supernatants or biological fluids may be tested; include matrix controls and validate assay performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive access to IVD raw materials, technical services, and consulting for diagnostic manufacturers and research laboratories. This NELL1 polyclonal antibody is part of our portfolio targeting bone biology and differentiation studies. We support your workflow with validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and expedited sourcing of rare-target raw materials to accelerate your assay development.