Anti-NELFE Polyclonal Antibody for WB, IF/ICC, ELISA - P18615

Core Product Specifications and Parameters

Biological Background: NELFE Function and Localization

• NELFE is a core subunit of the NELF complex, which negatively regulates transcription elongation by RNA polymerase II through transcriptional pausing that is counteracted by P-TEFb. Related references: PMID:10199401 PMID:27256882 PMID:11940650 PMID:12612062 PMID:18303858 PMID:27282391
• As the most potent RNA-binding component of NELF, NELFE is thought to mediate the initial recruitment of the complex to RNA. Related references: PMID:10199401 PMID:27256882 PMID:11940650 PMID:12612062 PMID:18303858 PMID:27282391
• The NELF complex is involved in HIV-1 latency, likely via recruitment of PCF11 to stalled RNA polymerase II.
• Located in the nucleus and associated with chromosomes, NELFE plays a direct role in transcriptional regulation at the chromatin level.
• NELFE is widely expressed across tissues, including heart, brain, lung, placenta, liver, skeletal muscle, kidney, and pancreas.
• The protein is subject to post-translational modifications such as ADP-ribosylation, phosphorylation, and ubiquitination, and contains a coiled-coil domain and repeat motifs.

Experimental Guidance and Technical Tips

• This rabbit polyclonal antibody targets the N-terminal region (aa 1–280) of human NELFE. For Western blot, anticipate a band near 43 kDa; validate specificity using siRNA knockdown or overexpression controls.
• In immunofluorescence assays, expect nuclear and chromosomal staining patterns. Include isotype controls and confirm localization with suitable markers.
• For ELISA, consider using recombinant NELFE fragments or native complexes for coating; optimize antibody dilution and detection conditions.
• Cross-reactivity is confirmed for human NELFE; reactivity with other species or isoforms should be verified experimentally if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a streamlined procurement experience for IVD raw materials, including high-quality antibodies against critical transcriptional regulators such as NELFE. Beyond this polyclonal antibody, we can support your projects with validated antibody pairs, optimized monoclonal options, and ancillary reagents for transcription research. Reach out to our team to discuss your specific sourcing needs for rare targets and custom IVD material pipelines.