Anti-NELFCD Polyclonal Antibody for WB, IHC-P, ELISA - Q8IXH7

Core Product Specifications and Parameters

Biological Background: NELFCD Function and Localization

• NELFCD encodes the negative elongation factor C/D (also known as TH1-like protein), a 66 kDa essential subunit of the NELF complex that regulates RNA polymerase II transcription elongation.
• The NELF complex associates with DSIF to induce promoter-proximal pausing of RNA polymerase II, a process reversed by P-TEFb kinase-mediated phosphorylation. Related references: PMID:10199401 PMID:12612062
• NELFCD is involved in HIV-1 latency, possibly by recruiting PCF11 to paused RNA polymerase II, highlighting a role in viral transcriptional regulation.
• The protein localizes to the nucleus and is widely expressed across tissues including heart, brain, lung, placenta, liver, skeletal muscle, kidney, and pancreas.
• Post-translational modifications and alternative splicing generate multiple isoforms, as indicated by keywords including alternative initiation, alternative splicing, and direct protein sequencing.

Experimental Guidance and Technical Tips

• For Western blotting, an expected band near 66 kDa should be observed; always validate in the relevant sample system (human, mouse, or rat) using appropriate loading controls.
• In IHC-P, nuclear staining is anticipated; optimization of antigen retrieval and antibody dilution may be necessary for consistent results.
• For ELISA applications, the antibody may be used as either capture or detection reagent; consider paired screening with other NELFCD antibodies to identify optimal combinations.
• The immunogen spans amino acids 40–182, a region conserved across human, mouse, and rat, supporting the reported cross-reactivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with one-stop access to IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. For transcription regulation studies, we supply high-quality NELFCD antibodies and can assist in custom raw-material procurement to accelerate your project from concept to clinic.