Anti-NEK9 Polyclonal Antibody for WB, IHC-P, ELISA - Q8TD19

Core Product Specifications and Parameters

Biological Background: NEK9 Function and Localization

• NEK9 (Nercc1 kinase, NimA-related kinase 9) is a serine/threonine kinase of the NEK family, playing a critical role in cell cycle regulation.
• It is a pleiotropic regulator of mitotic progression, essential for proper spindle dynamics and chromosome separation. Related references: PMID:12101123, PMID:12840024, PMID:14660563
• NEK9 phosphorylates histone H3 on serine and threonine residues, as well as beta-casein, myelin basic protein, and BICD2, suggesting broad substrate specificity. Related references: PMID:11864968
• The kinase is crucial for the G1/S transition and S phase progression, achieved through the phosphorylation and activation of downstream kinases NEK6 and NEK7. Related references: PMID:12840024, PMID:14660563, PMID:19941817
• NEK9 localizes to both the cytoplasm and nucleus, underscoring its involvement in mitotic spindle assembly and chromatin-associated functions.
• It is most highly expressed in heart, liver, kidney, and testis, with additional presence in smooth muscle cells and fibroblasts.
• Post-translational modifications include acetylation and phosphorylation; structural features comprise a coiled-coil domain and ATP-binding site.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 730–979) of human NEK9; this design may help reduce cross-reactivity with closely related kinases.
• For Western blotting, a predominant band at approximately 107 kDa is expected; consider using appropriate controls to confirm specificity.
• In immunohistochemistry (IHC-P), both nuclear and cytoplasmic staining patterns may be observed, reflecting the dual localization of NEK9.
• For ELISA applications, ensure that the recombinant immunogen or native protein is properly immobilized; validate the assay conditions in your specific sample matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic developers and research laboratories with streamlined access to premium IVD raw materials, including the NEK9 polyclonal antibody for cell cycle and mitosis studies. Our portfolio encompasses validated antibody pairs, monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. Partner with CamelBio to accelerate your assay development from concept to clinic.