Anti-NEK8 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q86SG6

Core Product Specifications and Parameters

Biological Background: NEK8 Function and Localization

• NEK8 (Never in mitosis A-related kinase 8) is a serine/threonine-protein kinase belonging to the NEK family of NIMA-related kinases.
• It is essential for renal tubular integrity and may regulate local cytoskeletal structure in kidney tubule epithelial cells.
• NEK8 may regulate ciliary biogenesis by targeting proteins to cilia. Related references: PMID:37598857
• Plays a role in organogenesis and is involved in the Hippo signaling pathway. Related references: PMID:26967905
• Predominantly localizes to the cytoplasm, and is associated with the cytoskeleton, centrosomes, and ciliary axonemes.
• Tissue expression is highest in thyroid, adrenal gland, and skin, with overexpression observed in breast tumors.
• As a phosphoprotein with ATP-binding activity, NEK8 is implicated in ciliopathies and nephronophthisis, highlighting its clinical relevance in kidney disease.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a region (aa 270–390) of human NEK8 outside the conserved kinase domain, potentially enhancing isoform specificity; validate in the relevant sample system.
• For Western blotting, the predicted molecular weight is ~75 kDa; ensure proper separation and transfer conditions to resolve the band clearly.
• In immunofluorescence applications, NEK8 localizes to the cytoplasm and cilia; consider co-staining with ciliary markers to confirm subcellular distribution.
• Cross-reactivity with mouse and rat is indicated; confirm performance using appropriate tissue lysates or cell lines from these species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines your IVD development by providing a one-stop platform for high-quality raw materials, including validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. This anti-NEK8 antibody supports nephrology and ciliopathy research, and our sourcing capabilities extend to rare targets and custom requirements. Partner with CamelBio to bridge the gap from concept to clinic with reliable, scalable supply solutions.