Anti-NEK7 Rabbit Monoclonal Antibody for WB, ELISA - Q8TDX7

Core Product Specifications and Parameters

Biological Background: NEK7 Function and Localization

• NEK7 (NIMA-related kinase 7) is a serine/threonine-protein kinase that plays an essential role in mitotic cell cycle progression, including centrosome microtubule nucleation, mitotic spindle formation, and cytokinesis.

Related references: PMID:17101132 PMID:19941817 PMID:17586473 PMID:19414596

• It phosphorylates downstream targets such as EML4 at Ser-146, promoting its dissociation from microtubules for efficient chromosome congression, and RPS6KB1.

Related references: PMID:31409757

• Beyond mitosis, NEK7 serves as a critical activator of the NLRP3 inflammasome, unlocking NLRP3 at the microtubule organizing center (MTOC) and enabling CASP1 activation independently of its kinase activity.

Related references: PMID:26642356 PMID:31189953 PMID:36442502 PMID:39173637

• NEK7 interaction with NEK9 enforces mutual exclusivity between inflammasome activation and cell division, acting as a cellular switch.

Related references: PMID:26642356 PMID:31189953

• Subcellularly, NEK7 localizes to the nucleus, cytoplasm, spindle poles, centrosomes, and MTOC.

• It is highly expressed in lung, muscle, testis, brain, heart, liver, leukocyte, and spleen, with lower expression in ovary, prostate, and kidney; absent in small intestine.

• Key structural and regulatory features include an ATP-binding kinase domain, metal-binding sites, and post-translational modifications such as acetylation and phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen covers a conserved region (aa 203-302) of human NEK7; expected to detect endogenous protein ~35 kDa in WB with human, mouse, and rat samples due to high sequence homology.
• For WB, consider using whole-cell lysates from commonly available cell lines (e.g., HeLa, HEK293T, Jurkat) as positive controls and validate with specific knockdown or knockout samples to confirm band identity.
• For ELISA, recombinant NEK7 protein or peptide can be used as coating antigen; titrate the antibody to determine optimal working concentration in your assay system.
• When studying inflammasome-related pathways, include appropriate activation treatments (e.g., LPS priming + nigericin) to assess NEK7’s role; note that its function in NLRP3 activation is kinase-independent.
• Given its nuclear and cytoplasmic localization, subcellular fractionation may be employed to enrich for specific compartments prior to detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with one-stop access to premium IVD raw materials, including this NEK7 monoclonal antibody for cell cycle and inflammasome studies. Beyond individual antibodies, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare-target raw materials, streamlining your development from concept to clinic.