Anti-NEK7 Polyclonal Antibody for WB, ELISA - Q8TDX7

Core Product Specifications and Parameters

Biological Background: NEK7 Function and Localization

• NEK7 (Never in mitosis A-related kinase 7) is a serine/threonine-protein kinase that plays a critical role in mitotic cell cycle progression (PubMed:17101132, PubMed:19941817, PubMed:31409757).
  Related references:
  PMID:17101132
  PMID:19941817
  PMID:31409757

• It is required for microtubule nucleation activity of the centrosome, robust mitotic spindle formation, and cytokinesis, and phosphorylates substrates such as EML4 and RPS6KB1 (PubMed:17586473, PubMed:19414596, PubMed:19941817).
  Related references:
  PMID:17586473
  PMID:19414596
  PMID:19941817

• Beyond mitosis, NEK7 acts as an essential activator of the NLRP3 inflammasome by unlocking NLRP3 after its translocation to the microtubule organizing center, independent of its kinase activity (PubMed:26642356, PubMed:31189953, PubMed:36442502, PubMed:39173637).
  Related references:
  PMID:26642356
  PMID:31189953
  PMID:36442502
  PMID:39173637

• NEK7 localizes to the nucleus, cytoplasm, spindle poles, and centrosome, and is highly expressed in tissues such as lung, muscle, testis, brain, heart, and liver.

• Post-translational modifications include acetylation and phosphorylation, consistent with its regulation in cell cycle and inflammatory signaling.

Experimental Guidance and Technical Tips

• The immunogen for this antibody corresponds to a recombinant fusion protein spanning amino acids 257-301 of human NEK7; consider this region when designing experiments or validating cross-reactivity.
• This polyclonal antibody is validated for WB and ELISA. For optimal results, titrate the antibody and validate in the relevant sample system.
• When investigating NEK7 function in mitotic progression or inflammasome activation, include appropriate controls and confirm antibody specificity in knockout or knockdown models if available.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a one-stop platform for IVD raw materials, including validated antibodies like this anti-NEK7 polyclonal antibody for kinase and inflammasome research. Beyond individual reagents, we offer integrated supply solutions encompassing validated antibody pairs, bulk ancillary reagents, and sourcing assistance for rare targets to support your entire development workflow—from concept to clinic.