Anti-NEK6 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q9HC98

Core Product Specifications and Parameters

Biological Background: NEK6 Function and Localization

• NEK6 (Serine/threonine-protein kinase Nek6), also designated NimA-related protein kinase 6 or SID6-1512, is a member of the NIMA-related kinase family.
• It is essential for mitotic cell cycle progression, playing roles in chromosome segregation at the metaphase-anaphase transition, robust mitotic spindle formation, and cytokinesis.
  Related references: PMID:11516946, PMID:14563848, PMID:19414596
• NEK6 phosphorylates numerous substrates including histones H1 and H3, KIF11, STAT3, EML4, and various ribosomal proteins, thereby regulating mitotic spindle dynamics, chromosome congression, and translation.
  Related references: PMID:12054534, PMID:20873783, PMID:19001501, PMID:31409757
• The kinase participates in G2/M phase cell cycle arrest triggered by DNA damage; inhibition of NEK6 activity leads to apoptosis.
  Related references: PMID:18728383
• NEK6 may support tumorigenesis by suppressing p53/TP53-induced cancer cell senescence.
  Related references: PMID:21099361
• Subcellularly, NEK6 localizes to the cytoplasm, nucleus, nuclear speckles, centrosomes, and spindle poles, mirroring its mitotic functions.
• It is ubiquitously expressed, with the highest levels found in heart and skeletal muscle.
• Additional keywords from UniProt include alternative splicing, apoptosis, ATP-binding, metal-binding, phosphoprotein, and serine/threonine-protein kinase, highlighting its regulatory complexity and post-translational control.

Experimental Guidance and Technical Tips

• The immunogen encompasses amino acids 200–300 of human NEK6, a region presumably within the kinase domain; verify sequence conservation across species to confirm predicted cross-reactivity with mouse and rat.
• For Western blotting, expect a band near 36 kDa; load positive control lysates from heart or skeletal muscle, where NEK6 is highly expressed.
• In IHC-P, both nuclear and cytoplasmic signals may be observed; include tissues with known NEK6 expression (e.g., heart, skeletal muscle) and negative controls to assess staining specificity.
• When developing an ELISA using this antibody, empirically optimize coating concentrations and blocking conditions to achieve the desired sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research labs to provide a complete portfolio of IVD raw materials, from validated antibody pairs to bulk ancillary reagents. This NEK6 Rabbit mAb supports investigations in cell cycle regulation, mitosis, and tumor biology. Our team also assists in sourcing rare-target raw materials and offers technical support to help move your projects from concept to clinic.