Anti-NEK3 Polyclonal Antibody for WB and ELISA - P51956

Core Product Specifications and Parameters

Biological Background: NEK3 Function and Localization

• Serine/threonine-protein kinase Nek3, also known as HSPK36 or NimA-related protein kinase 3.
• Influences neuronal morphogenesis and polarity by regulating microtubule acetylation in neurons.
• Mediates prolactin signaling in breast cancer cells, leading to phosphorylation of PXN and VAV2, and promoting RAC1 activation, cytoskeletal reorganization and cell motility.
• Subcellular localization includes the cytoplasm and cell projections such as axons.
• Up-regulated in malignant breast tissue; isoform 2 shows high expression in testis, ovary, and brain.
• Associated with keywords including acetylation, alternative splicing, ATP-binding, cell cycle, cell division, kinase, metal-binding, and phosphoprotein.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 267-506 of human NEK3; epitopes within this region are likely recognized.
• For Western blot, validate in relevant sample systems (e.g., human, mouse, or rat neuronal or breast cancer cell lines) and consider using fresh lysates.
• Typical starting dilutions should be optimized by the end user; perform a titration series to determine optimal working concentrations.
• For ELISA, test against the immunogen or recombinant NEK3 protein as a positive control.
• Note that polyclonal antibodies may exhibit batch-to-batch variability; validate each lot in your specific assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and researchers to accelerate IVD development by providing a comprehensive portfolio of raw materials, including validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. Our NEK3 polyclonal antibody supports research into neuronal morphogenesis and breast cancer signaling, with further supply capabilities for rare target sourcing and assay development. Contact our team to discuss your specific procurement needs and technical requirements.