Anti-NEK2 Monoclonal Antibody for WB, ELISA - P51955

Core Product Specifications and Parameters

Biological Background: NEK2 Function and Localization

• NEK2 is a serine/threonine-protein kinase, also known as HSPK 21, NimA-related protein kinase 2 (NimA-related protein kinase 2), and NimA-like protein kinase 1.
• It plays a critical role in centrosome separation and bipolar spindle formation during mitosis, ensuring high-fidelity chromosome segregation.
• NEK2 regulates kinetochore-microtubule attachment stability by phosphorylating NDC80, and controls the mitotic checkpoint via phosphorylation of CDC20 and MAD2L1.
• It phosphorylates centrosomal proteins such as CROCC, CEP250, NINL, CEP68, and CNTLN to promote their displacement from centrosomes, a process essential for centrosome disjunction. Related references: PMID:24554434, PMID:25704143, PMID:26220856
• NEK2 also phosphorylates CCDC102B, causing its dissociation from the centrosome and facilitating separation. Related references: PMID:30404835
• The protein is localized to the centrosome, spindle poles, kinetochores, and nucleus/nucleolus, reflecting its dynamic role throughout the cell cycle.
• Two major isoforms exist: isoform 1 and 2 are widely expressed, with isoform 4 found in testis; NEK2 is upregulated in various cancers.
• Post-translational modifications include phosphorylation, and the protein contains an ATP-binding domain and a coiled-coil region.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-100), which may detect multiple isoforms if the sequence is conserved.
• For Western blot, a band at approximately 52kDa is expected; optimal antibody dilution should be determined empirically.
• When using this antibody for ELISA, consider pairing it with a matched detection antibody for sandwich assays; validate performance in the relevant sample system.
• Cross-reactivity with mouse and rat NEK2 has been indicated; verify sequence homology between species to inform experimental design.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a one-stop supply of high-quality IVD raw materials, including validated antibody pairs, monoclonal/polyclonal antibodies, and ancillary reagents. This anti-NEK2 antibody supports cell cycle and oncology research, and CamelBio can assist with bulk orders, custom sourcing, and technical consultation to streamline your diagnostic development from concept to clinic.