Anti-NEDD9 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q14511

Core Product Specifications and Parameters

Biological Background: NEDD9 Function and Localization

• NEDD9, also known as HEF1, CAS-L, and CASS2, is a scaffolding protein belonging to the Cas family, characterized by multiple protein interaction domains (SH3 domain) and a C-terminal domain involved in focal adhesion targeting.
• Functions as a central coordinator of tyrosine kinase signaling at focal adhesions, regulating cell adhesion, migration, and cytoskeletal reorganization. Related references: PMID:24574519
• Mediates integrin β1 and B-cell receptor signaling in lymphocytes, promoting lymphocyte adhesion and migration, and is required for proper lymphocyte homing to secondary lymphoid organs. Related references: PMID:9020138 PMID:17174122
• Plays a role in T-cell immunological synapse formation by organizing the cortical actin cytoskeleton and centralizing TCR microclusters.
• Negatively regulates ciliogenesis and promotes cilia disassembly via an AURKA/HDAC6-dependent pathway that deacetylates α-tubulin. Related references: PMID:17604723
• Localizes to multiple subcellular compartments including focal adhesions, lamellipodia, the Golgi apparatus, nucleus, spindle poles, and the basal body of cilia, reflecting its versatile roles in signaling.
• Exhibits alternative splicing and is regulated by phosphorylation and ubiquitination, as indicated by the presence of phosphoprotein and Ubl conjugation keywords.
• Shows high expression in kidney, lung, placenta, and lymphocytes, with protein expression detected in B-cells and respiratory epithelium. Related references: PMID:9020138 PMID:9584194

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 390–560, located in the central region of NEDD9; epitopes may be preserved across human, mouse, and rat due to high homology.
• For Western blotting, expect a band around 93 kDa; however, alternative splicing and post-translational modifications may result in multiple bands.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), fixation and antigen retrieval conditions should be optimized; nuclear and cytoplasmic localization may be observed.
• For ELISA, the recombinant protein used as immunogen can serve as a positive control; note that the polyclonal nature may cause batch-to-batch variability.
• Cross-adsorption may be considered if cross-reactivity with other Cas family proteins (BCAR1, EFS) needs to be minimized.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with a streamlined supply chain for IVD raw materials. This anti-NEDD9 polyclonal antibody is available along with complementary reagents for cell adhesion and migration studies. We also offer customized solutions for rare target antibody development and bulk production to support your development pipeline.