Anti-NEDD8 Rabbit Polyclonal Antibody for WB and ELISA - Q15843

Core Product Specifications and Parameters

Biological Background: NEDD8 Function and Localization

• NEDD8 is a ubiquitin-like protein (also known as neddylin) that shares structural similarity with ubiquitin and is a key component of the neddylation pathway. It is encoded by the NEDD8 gene in humans (GeneID: 4738).
• NEDD8 plays a critical role in cell cycle control and embryogenesis. Conjugation of NEDD8 to cullins (neddylation) is required for the recruitment of E2 enzyme to the cullin-RING E3 ubiquitin ligase complex, facilitating the polyubiquitination and subsequent proteasomal degradation of cyclins and other regulatory proteins.
  Related references:
  PMID:10318914, PMID:10597293, PMID:11953428
• Neddylation of the tumor suppressor p53/TP53 inhibits its transcriptional activity, linking NEDD8 to p53 regulation.
  Related references:
  PMID:15242646
• NEDD8 also neddylates the epidermal growth factor receptor (EGFR), resulting in protein stabilization and translocation to the cell membrane.
  Related references:
  PMID:28891468
• Covalent attachment of NEDD8 to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M.
  Related references:
  PMID:14690597
• NEDD8 is primarily localized in the nucleus, consistent with its role in cell cycle and transcriptional regulation.
• It is highly expressed in heart, skeletal muscle, spleen, thymus, prostate, testis, ovary, colon, and peripheral blood leukocytes.
• Post-translational modifications of NEDD8 include acetylation and the formation of isopeptide bonds during neddylation. The mature protein has a molecular weight of ~9 kDa.

Experimental Guidance and Technical Tips

• The immunogen spans the full-length NEDD8 protein (aa 1-81), so the antibody likely detects the mature 9 kDa form. Consider using high-percentage gels (e.g., 15% SDS-PAGE) and appropriate low-molecular-weight markers for Western blot.
• The antibody is reported for WB and ELISA applications; suitability for other techniques such as IHC or IF may require experimental validation.
• Cross-reactivity with mouse and rat NEDD8 is indicated, but it is recommended to verify reactivity in your specific experimental system.
• For ELISA, consider using the antibody as both capture and detection in a sandwich format, but optimization of coating and detection conditions is advised.
• Store the antibody as recommended (shipped on ice bags), and avoid repeated freeze-thaw cycles to maintain stability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials. For ubiquitin-like protein targets such as NEDD8, we offer validated polyclonal antibodies like this NEDD8 Rabbit pAb, along with complementary reagents and custom sourcing services to support your assay development from concept to clinic. Whether you need bulk quantities, matched antibody pairs, or assistance with rare target procurement, CamelBio is your reliable partner.