Anti-NEDD8 Rabbit Monoclonal Antibody for WB, ELISA - Q15843

Core Product Specifications and Parameters

Biological Background: NEDD8 Function and Localization

• NEDD8 is a ubiquitin-like protein that conjugates to cullins and p53/TP53, essential for cell cycle control and embryogenesis. Its attachment to cullins enables E2 recruitment to the cullin-RING E3 ligase complex, facilitating polyubiquitination and proteasomal degradation of cyclins and regulatory proteins. Related references: PMID:10318914 PMID:10597293 PMID:11953428
• NEDD8 conjugation to p53/TP53 inhibits its transcriptional activity. Related references: PMID:15242646
• Activation of NEDD8 requires the E1 complex UBE1C-APPBP1 and the E2 enzyme UBE2M. Related references: PMID:14690597
• NEDD8 also neddylates EGFR, stabilizing the protein and promoting its translocation to the cell membrane. Related references: PMID:28891468
• Subcellular localization is primarily nuclear.
• Highly expressed in heart, skeletal muscle, spleen, thymus, prostate, testis, ovary, colon, and peripheral blood leukocytes.
• Post-translational modifications include acetylation and isopeptide bond formation, with a molecular weight of approximately 9 kDa (UniProt: 9072 Da).

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein covering the N-terminal 81 amino acids of human NEDD8 (NP_006147.1), a sequence highly conserved across human, mouse, and rat, ensuring broad cross-reactivity.
• For Western blot analysis, note the ~9 kDa mass; use a high-percentage (15%) gel and optimize protein transfer conditions to prevent over-run, and validate performance in relevant cell or tissue lysates.
• For ELISA, pair with a matched detection antibody or use as a detection reagent; empirical optimization of coating and detection conditions is recommended.
• Although validated for human, mouse, and rat, cross-reactivity with other species should be verified by sequence alignment of the immunogen region.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your one-stop sourcing partner for IVD raw materials, offering this anti-NEDD8 Rabbit mAb for research in ubiquitin-like pathways and cell cycle control. Beyond this product, we provide validated antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets, supporting seamless development from concept to clinic.