Anti-NEDD4L Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q96PU5

Core Product Specifications and Parameters

Biological Background: NEDD4L Function and Localization

• E3 ubiquitin-protein ligase that mediates the polyubiquitination of lysine and cysteine residues on target proteins, thereby regulating autophagy, innate immunity, and DNA repair.
  Related references:
  PMID:20064473
  PMID:31959741
  PMID:33608556

• Inhibits TGF-beta signaling by triggering ubiquitination and proteasome-dependent degradation of SMAD2 and TGFBR1.
  Related references:
  PMID:15496141

• Downregulates autophagy and cell growth by ubiquitinating and reducing cellular ULK1 or ASCT2 levels.
  Related references:
  PMID:28820317
  PMID:31959741

• Promotes ubiquitination and internalization of various plasma membrane channels (e.g., ENaC, SCN2A, SCN3A, SCN5A, SCN9A, SCN10A, KCNA3, KCNH2, EAAT1, KCNQ2, KCNQ3, CLC5).
  Related references:
  PMID:26363003
  PMID:27445338

• Plays a role in antiviral innate immunity by catalyzing 'Lys-29'-linked cysteine ubiquitination of TRAF3, enhancing further ubiquitination.
  Related references:
  PMID:33608556

• Ubiquitinates TTYH2 and TTYH3 and regulates protein levels of TTYH2.
  Related references:
  PMID:18577513

• Localizes to the cytoplasm, Golgi apparatus, endosome, and multivesicular body.

• Ubiquitously expressed, with highest levels in prostate, pancreas, and kidney; also expressed in melanocytes.
  Related references:
  PMID:14615060
  PMID:15496141
  PMID:19664597
  PMID:23999003

• Keywords: alternative splicing, phosphoprotein, disease variant, host-virus interaction, differentiation, and more, reflecting its multifaceted roles.

Experimental Guidance and Technical Tips

• This antibody was raised against a recombinant fragment corresponding to amino acids 836–955 of human NEDD4L. Consider validating its ability to detect full-length endogenous NEDD4L and potential isoforms in your model system.
• For Western blot (WB), a band around 112 kDa is expected; optimize loading controls and transfer conditions for high molecular weight proteins.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), pre-adsorption or appropriate controls can help confirm signal specificity, given the polyclonal nature.
• The antibody may cross-react with mouse and rat NEDD4L, but cross-species reactivity should be verified with appropriate controls.
• ELISA detection requires optimization of coating antigen and antibody dilution; the immunogen sequence can guide antigen design.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers, laboratories, and research institutes from concept to clinic. Our portfolio includes validated antibody pairs, optimized polyclonal antibodies like this anti-NEDD4L, and bulk ancillary reagents. Whether you need rare-target raw materials for ubiquitination or signaling studies, CamelBio streamlines procurement to accelerate your assay development.