Anti-NEDD4 Polyclonal Antibody for WB, IHC-P, ELISA - P46934

Core Product Specifications and Parameters

Biological Background: NEDD4 Function and Localization

• NEDD4 (neural precursor cell expressed developmentally down-regulated protein 4) is also known as RPF1, NEDD4-1, and cell proliferation-inducing gene 53 protein. It is a HECT-type E3 ubiquitin ligase that plays critical roles in protein degradation, receptor signaling, and autophagy.
• As an E3 ubiquitin ligase, NEDD4 accepts ubiquitin from E2 enzymes and specifically transfers ubiquitin to lysine-63 of target substrates, forming polyubiquitin chains. Related references: PMID:19920177, PMID:21399620, PMID:23644597
• NEDD4 ubiquitinates a wide range of substrates, including growth factor receptors (IGF1R, FGFR1, EGFR), signaling proteins (RAPGEF2, TNK2, BRAT1, DAZAP2), and transcriptional machinery (POLR2A), targeting many for internalization and lysosomal or proteasomal degradation. Related references: PMID:21765395, PMID:11598133, PMID:20086093, PMID:25631046, PMID:11342538, PMID:19920177
• The role of NEDD4 in regulating PTEN stability through polyubiquitination has been debated; some studies suggest a direct link, while others question it. Related references: PMID:18562292, PMID:17218260
• NEDD4 functions as a platform to recruit USP13, forming a deubiquitination complex that cleaves Lys-48-linked ubiquitin chains from VPS34, stabilizing VPS34 and promoting autophagosome formation. Related references: PMID:32101753
• During viral infection, NEDD4 ubiquitinates Ebola virus VP40, contributing to viral budding.
• NEDD4 localizes to the cytoplasm, nucleus, and cell membrane, and undergoes post-translational modifications including acetylation and phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 160-460, a central region of human NEDD4, suggesting the antibody may recognize multiple isoforms or functional domains; validation in the intended sample system is recommended.
• For western blotting, the predicted molecular weight is approximately 149 kDa; consider using a lower percentage gel (e.g., 6-8%) and extended transfer times to ensure efficient transfer of high-molecular-weight proteins.
• In immunohistochemistry (paraffin-embedded tissues), antigen retrieval conditions may need optimization; include appropriate positive and negative controls to confirm specificity.
• For ELISA, the polyclonal nature can provide high avidity; assess performance as both capture and detection antibody, and validate against relevant recombinant standards.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a streamlined, one-stop channel for high-quality IVD raw materials. For researchers exploring ubiquitination pathways, receptor degradation, or autophagy, CamelBio offers a portfolio that includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and tailored sourcing for rare targets. We are dedicated to supporting your project from concept to clinic with reliable supply and technical expertise.