Anti-NEDD4 Monoclonal Antibody for WB, ELISA - P46934

Core Product Specifications and Parameters

Biological Background: NEDD4 Function and Localization

• NEDD4 (Neural precursor cell expressed developmentally down-regulated protein 4) is a HECT-type E3 ubiquitin-protein ligase that transfers ubiquitin to target proteins, primarily catalyzing Lys-63-linked polyubiquitination. Related references: PMID:19920177 PMID:21399620 PMID:23644597
• It regulates receptor tyrosine kinases by monoubiquitinating IGF1R and FGFR1, leading to their internalization and lysosomal degradation. Related references: PMID:21765395
• NEDD4 ubiquitinates key signaling molecules including VEGFR-2/KDFR, RAPGEF2, ERBB4 intracellular domain, TNK2, and POLR2A, impacting processes such as neuronal dendrite development, EGFR signaling, and transcriptional regulation. Related references: PMID:11598133 PMID:20086093 PMID:19920177
• It forms a deubiquitination complex with USP13 to cleave Lys-48-linked ubiquitin chains from VPS34, stabilizing VPS34 and promoting autophagosome formation. Related references: PMID:32101753
• Subcellularly, NEDD4 localizes to the cytoplasm, nucleus, and cell membrane, consistent with its roles in endocytosis, nuclear signaling, and membrane-associated protein turnover.
• Alternative splicing generates multiple isoforms, and post-translational modifications include acetylation and phosphorylation, which may regulate its activity and interactions.
• During viral infection, NEDD4 ubiquitinates Ebola virus VP40 protein, facilitating viral budding.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1200–1300 within the C-terminal region of human NEDD4. Confirm the antibody’s reactivity with specific isoforms if multiple variants are present in the target sample.
• For Western blotting, NEDD4 has a predicted molecular weight of ~149 kDa. Use appropriate gel percentage (e.g., 6–8% SDS-PAGE) and prolonged transfer times to ensure efficient blotting of high-molecular-weight proteins.
• Validate the antibody in your sample system using positive controls (e.g., HEK293 or HeLa lysates) to ensure specificity and sensitivity for the intended application.
• For ELISA, consider optimizing coating and detection conditions to achieve maximal signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with a comprehensive range of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. For NEDD4 research, we offer this high-quality monoclonal antibody suitable for WB and ELISA applications, facilitating your investigations into ubiquitination pathways, autophagy, and related diseases. Explore our full portfolio to streamline your assay development from concept to clinic.