Anti-NEDD1 Polyclonal Antibody for WB, IF/ICC, ELISA - Q8NHV4

Core Product Specifications and Parameters

Biological Background: NEDD1 Function and Localization

• NEDD1, also known as Neural precursor cell expressed developmentally down-regulated protein 1 (NEDD-1), is a centrosomal protein encoded by the NEDD1 gene in humans.
• It plays a critical role in mitosis by promoting the nucleation of microtubules from the spindle, which is essential for proper chromosome segregation.
• NEDD1 localizes primarily to the centrosome, the major microtubule-organizing center in animal cells, and is found in the cytoplasm and associated with the cytoskeleton.
• The protein contains WD repeats, which are structural motifs involved in protein-protein interactions, suggesting it functions as a scaffold in centrosomal complexes.
• NEDD1 is subject to alternative splicing and phosphorylation, indicating regulatory mechanisms that may control its activity during the cell cycle.
• As a key mitotic regulator, NEDD1 is implicated in cell division and cycle control, and is part of the reference proteome for human.

Experimental Guidance and Technical Tips

• For Western blotting, the expected molecular weight of NEDD1 is approximately 72 kDa; consider using a positive control lysate from human, mouse, or rat cells.
• In immunofluorescence/ICC, NEDD1 should localize to the centrosome and mitotic spindle; validate staining patterns in dividing cells to confirm specificity.
• The immunogen corresponds to amino acids 411-660; for ELISA, consider using recombinant NEDD1 fragments or full-length protein as coating antigen to assess antibody binding.
• When using this polyclonal antibody, include appropriate negative controls (e.g., pre-immune serum or blocking peptide) to rule out non-specific signals, especially in IF applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated partner for IVD raw materials, providing a reliable supply of validated antibody pairs, monoclonal and polyclonal antibodies, and ancillary reagents. This anti-NEDD1 polyclonal antibody supports research in mitosis and centrosome biology, and we can assist with bulk orders or custom sourcing for targets like NEDD1 and other cell cycle regulators. Whatever your development stage, from concept to clinic, CamelBio streamlines your raw-material procurement with a comprehensive, one-stop approach.