Anti-Nectin-4/PVRL4 Rabbit Monoclonal Antibody for FC, ELISA - Q96NY8

Core Product Specifications and Parameters

Biological Background: Nectin-4 Function and Localization

• Nectin-4 (also known as PVRL4, LNIR, or PRR4) is a member of the nectin family of immunoglobulin-like cell adhesion molecules, encoded by the NECTIN4 gene.
• It mediates cell adhesion through trans-homophilic and trans-heterophilic interactions, particularly with NECTIN1, but does not act as a receptor for alpha-herpesvirus entry.
• Nectin-4 serves as the primary receptor for measles virus, facilitating viral entry into host cells.
• Subcellular localization includes the cell membrane, adherens junctions, and secretion into the extracellular space.
• Predominantly expressed in placenta; notably absent in normal breast epithelium but highly expressed in breast carcinoma, suggesting a role in tumor progression.
• Post-translational modifications include N-linked glycosylation and disulfide bond formation, essential for proper folding and function.
• Alternative splicing generates isoforms that may vary in function and localization.
• Genetic variants in NECTIN4 are associated with ectodermal dysplasia syndromes, highlighting its developmental importance.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular Ig-like domain (aa 32-146), making this antibody suitable for detecting surface-expressed Nectin-4 in flow cytometry (FC) without permeabilization.
• For ELISA, consider using the antibody as a capture or detection reagent; validate against recombinant Nectin-4 protein and native protein in relevant cell lysates or supernatants.
• Given the dual protein weights (24 kDa and 55 kDa), verify which isoform is expressed in your experimental system by western blot if adapted for such use.
• In measles virus entry assays, confirm that antibody binding does not interfere with virus-receptor interaction if studying neutralization.
• For breast carcinoma studies, include normal breast tissue as a negative control due to the differential expression pattern.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and researchers with a comprehensive portfolio of IVD raw materials, including optimized monoclonal antibodies like this anti-Nectin-4 clone for virology and oncology applications. We offer validated antibody pairs, bulk ancillary reagents, and rare target sourcing to streamline your assay development from concept to clinic. Let CamelBio be your trusted partner in advancing Nectin-4-related diagnostic and therapeutic projects.